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ID   PMA200U    preliminary; circular DNA; SYN; 4152 BP.
AC   M36426; ATCC37833;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phagemid vector pMA200U - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCE30 from pUC9 & pMY17-3, lambda cI857 gene/pR/pL
RC   pCE33 from pCE30 & pMOB45
RC   pND201 from pCE30 & linker
RC   pPT150 from pCE30 & pBR322
RC   pMA200U from pCE30 & pTZ18U
RA   Elvin C.M., Thompson P.R., Argall M.E., Hendry P., Stamford N.P.,
RA   Lilley P.E., Dixon N.E.;
RT   "Modified bacteriophage lambda promoter vectors for overproduction
RT   of proteins in Escherichia coli";
RL   Gene 87:123-126(1990).
CC   Constructed by replacing the 1.118 kb BglI fragment of pCE30 with the
CC   analogous 1.27 kb fragment of pTZ18U.
CC   Upon infection with M13KO7 (ATCC 37468), an M13-sensitive host strain
CC   containing a pMA200U derivative produces packaged single-stranded
CC   plasmid DNA complementary to the M13 universal primer.
CC   If the insert is oriented correctly for transcription from the lambda
CC   promoters, the single-stranded plasmid DNA will contain the coding
CC   strand.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.3; BamHI/EcoRI--4.3; BglI--3.0, 1.3. (ATCC staff)
CC   Expression vector encoding cI857.  Contains primer sites useful for
CC   sequencing and permits production of ssDNA. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMA200U)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   HO (E.coli AN1459)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pCE30 remove BglI-BglI 1118bp,
FT                   \ pUC9 416..1534/2883bp
FT                   2. pTZ18U BglI-BglI 1268bp 462..1730
FT                   -> pMA200U 4152bp"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-SmaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PL"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PR"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;