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ID   PMC1585    preliminary; circular DNA; SYN; 8600 BP.
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AC   IG7023;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pMC1585 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pMC1513 from pMC1403 & pKB252, lacUV5 promoter
RC   pSKS104 from pMC1403 & M13mp7
RC   pSKS105 from pMC1403 & M13mp8
RC   pSKS106 from pMC1403 & M13mp9
RC   pMC1585 from pMC1403 & yeast 2 micron & URA3 gene
RC   pMC1587 from pMC1403 & yeast 2 micron & LEU2 gene
RC   pMC1790 from pMC1403 & ARS1 & TRP1 gene
RC   pMC2010 from pMC1403 & yeast 2 micron & TRP1 gene
RC   pMC931 from pMC1403 & pACYC177
RC   pMC1871 from pMC1403 & M13mp7
RC   [pMC1364 from pMC series]
RC   [pMC1396 from pMC series]
RC   [pMC1511 from pMC series]
RC   [pMC1571 from pMC1511]
RC   [pMC1981 from pMC1871]
RA   Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J.;
RT   "Beta-galactosidase gene fusions for analyzing gene expression in
RT   Escherichia coli and yeast";
RL   Meth. Enzymol. 100:293-308(1983).
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CC   NM (pMC1585)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E. coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(yeast 2 micron)
CC   BR (pMC1364)(pMC1396)(pMC1403)(pMC1511)(pMC1843)(pMC1844)(pMC13871)
CC   OF (pGSR1 with URA3 and 2 micron plasmid ori)
CC   OR (yes)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMC1403 remove, 9820bp-
FT                   2. yeast 2 micron, ori
FT                   3. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> pMC1585 8600bp"
FT   misc_feature    0..0
FT                   /note="BamHI"
FT   misc_feature    0..0
FT                   /note="PvuII"
FT   misc_feature    0..0
FT                   /note="pBR322 ori"
FT   misc_feature    0..0
FT                   /note="pBR322 amp"
FT   misc_feature    0..0
FT                   /note="EcoLac"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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