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ID   PMF7       preliminary; circular DNA; SYN; 6800 BP.
AC   ATCC37117;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli cosmid vector pMF7 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pSF1 from pBR322 & lambda
RC   pBW3 from pBR322 & lambda-21 hy 33
RC   pMF517 from pBR322 & Charon 4A, cos
RC   pBW8 from pBR322 & lambda, cos2
RC   pBW89 from pBR322 & Charon 4A
RC   pMF1, pMF7 from pBR322 & phi80
RC   pDS15, pDS16, pMF22 from pBR322 & lambda
RA   Feiss M., Siegele D.A., Rudolph C.F., Frackman S.;
RT   "Cosmid DNA packaging in vivo";
RL   Gene 17:123-130(1982).
RN   [2]
RC   pBW3 from pBR322 & phi21, cos
RC   pMF1 from pBR322 & lambda, cos
RC   pMF2 from pMF1
RC   pMF517 from pBR322 & lambda, cos
RC   pGM2 from pDS15 & lambda, cI857
RC   pBW5 from pBW3 & lambda, cI857
RC   pBW6 from pDS15 & lambda 21
RA   Feiss M., Widner W.;
RT   "Bacteriophage lambda DNA packaging: scanning for the terminal
RT   cohesive end site during packaging";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:3498-3502(1982).
RN   [3]
RC   pDS15 from pBR322 & lambda, cos
RC   pGM1, pGM2, pGM4, pGM5 from pDS15 & lambda, cI857
RA   Miller G., Feiss M.;
RT   "Cohesive end annealing and the helper-mediated transformation
RT   system of phage lambda";
RL   Virology 109:379-390(1981).
CC   A general purpose cosmid vector. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMF7)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli IC202)(E.coli)(E.coli K802 recA-)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-BamHI 377bp
FT                   \ 4360..4361..376, 3984bp
FT                   2. lambda 2800bp 47190..48502..140,
FT                   \ lambda cos 97.3% to 0.3%
FT                   -> pMF7 6800bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;