ID   PMM226     preliminary; circular DNA; SYN; 457 BP.
XX
AC   M19081; M19082; M19084;
XX
DT   15-JUN-1988 (Rel. 3, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMM2-26 - incomplete,pHS1/Tn3#2 3 pMB8/Tn3#2 5.
XX
KW   cloning vector; Tn3; sequence F; sequence G; sequence H.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-95, F
RP   1-84, G
RP   1-97, H
RC   pMB8::Tn3#2, pMB8::Tn3#5 from pMB8 & Tn3
RC   pMM5-3 from pHS1 & pMB8::Tn3#5
RC   pMM2-26, pMM2-32, pMM2-34 from pHS1 & pMB8::Tn3#2
RA   McCormick M., Ohtsubo E.;
RT   "Cointegrates carrying two copies of a Tn3 derivative in an inverted
RT   orientation";
RL   Gene 34:197-206(1985).
XX
CC   NCBI gi: 207818 & 207819 & 207820
CC   NM (pMM2-26)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pHS1)(Tn3)(pMB8)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHS1 EcoRI
FT                   remove 274bp
FT                   2. pMB8::Tn3#2 EcoRI-EcoRI, Tn3#2
FT                   3. pMB8::Tn3#2 EcoRI-EcoRI, Tn3#2
FT                   -> pMM2-26"
FT   misc_recomb     43..44
FT                   /note="Tn3 DNA end/pHS1 DNA start; sequence F"
FT   misc_recomb     37..38
FT                   /note="pHS1 DNA end/Tn3 DNA start; sequence G"
FT   misc_recomb     43..44
FT                   /note="Tn3 DNA end/pMB8 DNA start; sequence H"
XX
SQ   Sequence 457 BP; 57 A; 73 C; 74 G; 72 T; 181 other;
     aatcccttaa cgtgagtttt cgttccactg agcgtcagac ccctttacgg gcggctctgg
     cttcggctcg cttttctgcc tgtatcaggt tgcgcnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     ccggcagact ggtacagagc atggtcacgg gctttacggg gtctgacgct cagtggaacg
     aaaactcacg ttaagggatt gatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     cgttgcttaa cgtgagtttt cgttccactg agcgtcagac ccctatactg gcttaactaa
     tacggttatc cacagaatca ggggataacg cagggcc
//