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ID   PMMB33     preliminary; circular DNA; SYN; 13750 BP.
AC   ATCC37248;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli cosmid vector pMMB33 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pMMB7 from RSF1010 & linker
RC   pMMB31 from pKT231 & pHC79
RC   pMMB33, pMMB34 from pMMB7 & pMMB31
RA   Frey J., Bagdasarian M., Feiss D., Franklin F.C., Deshusses J.;
RT   "Stable cosmid vectors that enable the introduction of cloned
RT   fragments into a wide range of gram-negative bacteria";
RL   Gene 24:299-308(1983).
RN   [2]
RC   R1, R136, R192, R538-1, R538-2, R64, R144, R163 from E.coli
RC   R1drd-10, R1drd-14, R1drd-16, R1drd-19 from R1
RC   R136drdH8 from R136
RC   R192drdF7 from R192
RC   R538-1drd from R538-1
RC   R538-2drd from R538-2
RC   R64drd11 or R64.11 from R64
RC   R144drd3 from R144
RC   R163drdE6 from R163
RC   [R1-19, R1-19K- from R1]
RA   Meynell E., Cooke M.;
RT   "Repressor minus and operator constitutive derepressed mutants of
RT   F like R factors. Their effect on chromosomal transfer by HfrC";
RL   Genet. Res. 14:309-313(1969).
RN   [3]
RC   pLG221 from ColIdrd-1 & Tn5
RC   pLG223 from ColIdrd-1 & Tn10
RC   pLG281 from pBR325 & ColIdrd-1
RC   pLG282 from pBR325 & ColIdrd-1
RA   Boulnois G.J.;
RT   "Colicin Ib does not cause plasmid-promoted abortive phage
RT   infection of Escherichia coli K-12";
RL   Mol. Gen. Genet. 182:508-510(1981).
RN   [4]
RC   pJD1 from Pseudomonas JD1
RC   pJD2 from pJD1 & Pseudomonas myo-inositol transport gene
RC   pJD3 from pJD1 & Pseudomonas myo-inositol transport gene
RA   Gauchat-Feiss D., Frey J., Belet M., Deshusses J.;
RT   "Cloning of genes involved in myo-inositol transport in a
RT   Pseudomonas sp";
RL   J. Bacteriol. 162:324-327(1985).
RN   [5]
RC   R68.45 from E. coli
RA   Willets N.S., Crowther C., Holloway B.W.;
RT   "The insertion sequence IS21 of R68.45 and the molecular basis
RT   for mobilization of the bacterial chromosome";
RL   Plasmid 6:30-52(1981).
RN   [6]
RC   ColIdrd-1 from ColIb
RA   Wilkins B.M., Boulnois G.J., Lanka E.;
RT   "A plasmid DNA primase active in discontinuous bacterial DNA
RT   replication";
RL   Nature 290:217-221(1981).
CC   A broad host range cosmid vector.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pMMB33)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli C600)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. RSF1010 HpaI 8684bp 1..1
FT                   2. linker 9bp ccggatcgg
FT                   -> pMMB7 8693bp [BamHI in sulf/spec genes]
FT                   1. E. coli
FT                   -> R6-5
FT                   1. R6-5, kan gene
FT                   -> pKT105
FT                   1. RSF1010 8684bp
FT                   2. pKT105 1300bp, kan gene
FT                   -> pKT231 10000bp
FT                   1. pKT231 BamHI 10000bp
FT                   2. pHC79 BglII-BglII 1719bp 2111..3830, lambda cos
FT                   -> pMMB31 11700bp
FT                   1. pMMB7 PstI 8693bp, sulf/spec/RSF1010 7773 or 8573
FT                   2. pMMB31 PstI-PstI 5000bp, lambda cos
FT                   \ RSF1010 7773 or 8573
FT                   -> pMMB33 13750bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SstI-EcoRI-HpaI"
FT   rep_origin      0..0
FT                   /note="ORI Pseudomonas R300B"
FT   CDS             0..0
FT                   /note="ANT Pseudomonas kanamycin resistance gene
FT                   (kan)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;