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ID   PMMB34     preliminary; circular DNA; SYN; 13750 BP.
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AC   ATCC37249;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Broad host range/E.coli cosmid vector pMMB34 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pMMB7 from RSF1010 & linker
RC   pMMB31 from pKT231 & pHC79
RC   pMMB33, pMMB34 from pMMB7 & pMMB31
RA   Frey J., Bagdasarian M., Feiss D., Franklin F.C., Deshusses J.;
RT   "Stable cosmid vectors that enable the introduction of cloned
RT   fragments into a wide range of gram-negative bacteria";
RL   Gene 24:299-308(1983).
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CC   A broad host range cosmid vector.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pMMB34)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli C600)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. RSF1010 HpaI 8684bp 1..1
FT                   2. linker 9bp ccggatcgg
FT                   -> pMMB7 8693bp [BamHI in sulf/spec genes]
FT                   1. E. coli
FT                   -> R6-5
FT                   1. R6-5, kan gene
FT                   -> pKT105
FT                   1. RSF1010 8684bp
FT                   2. pKT105 1300bp, kan gene
FT                   -> pKT231 10000bp
FT                   1. pKT231 BamHI 10000bp
FT                   2. pHC79 BglII-BglII 1719bp 2111..3830, lambda cos
FT                   -> pMMB31 11700bp
FT                   1. pMMB7 PstI 8693bp, sulf/spec/RSF1010 7773 or 8573
FT                   2. pMMB31 PstI-PstI 5000bp, lambda cos
FT                   \ RSF1010 7773 or 8573
FT                   -> pMMB34 13750bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SstI-EcoRI-HpaI"
FT   rep_origin      0..0
FT                   /note="ORI Pseudomonas R300B"
FT   CDS             0..0
FT                   /note="ANT Pseudomonas kanamycin resistance gene
FT                   (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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