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ID   PMP78      preliminary; circular DNA; SYN; 8700 BP.
AC   ATCC37179;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pMP78 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pMP78-1, pMP80-3 from pBR325 & pJDB219, yeast 2 micron/LEU2 gene
RC   pMP81 from pCR1 & pJDB219, yeast 2 micron/LEU2 gene
RA   Hollenberg C.P.;
RT   "The expression in Saccharomyces cerevisiae of bacterial
RT   beta-lactamase and other antibiotic resistance genes integrated in
RT   a 2 micron DNA vector";
RL   ICN-UCLA Symp. Mol. Cell. Biol. [Extrachrom DNA] 15:325-338(1979).
RL   Cummings D.J., Borst P., Dawid I.B., Weissman S.M., Fox C.F.;
RL   Academic Press, New York.
CC   A general purpose episomal Saccharomyces cerevisiae/E.coli
CC   shuttle vector. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMP78)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli JA221)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pMB9 EcoRI 5288bp 5287..5287, 5' to tet gene
FT                   2. yeast 2 micron B form EcoRI-EcoRI 2235bp
FT                   \ 2408..3942 & 703..2
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   -> pJBD41 7523bp [tet gene]
FT                   1. pJBD41 PstI 7523bp, amp gene
FT                   \ yeast 2 micron 2657..2657
FT                   dAT tailing
FT                   2. yeast 1200bp, LEU2 gene
FT                   sheared
FT                   dAT tailing
FT                   -> pJDB219 8700bp [HindIII-HindIII 3600bp, ori/leu2]
FT                   1. pBR325 HindIII 5994bp 30..30
FT                   2. pJDB219 HindIII-HindIII 3300bp, yeast 2 micron/LEU2
FT                   -> pMP78-1 8700bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;