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ID   PMPOT2     preliminary; circular DNA; SYN; 1 BP.
AC   ATCC20744;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pMPOT2 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pMPOT2 from yeast 2 micron & Schizosaccharomyces pombe
RA   Lin K.Y., Matteucci M.;
RT   "Hybridization properties of deoxyoligonucleotides containing
RT   anthraquinone pseudonucleosides";
RL   Nucleic Acids Res. 19:3111-3111(1990).
CC   A vector which contains REP1, REP2, REP3 and ori sequences from
CC   yeast 2 micron plasmid and the triose phosphate isomerase gene from
CC   Schizosaccharomyces pombe (POT1).  POT1 provides for plasmid
CC   maintenance in triose phosphate deficient hosts. [1]
CC   Note: This material is cited in a U.S and/or other Patent and may not
CC   be used to infringe the patent claims.
CC   Growth media: YEPD (ATCC medium number 1245) 24C
CC   Deposited by: Zymogenetics, Inc.
CC   NM (pMPOT2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)(Zymogenetics)
CC   HO (Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)(episomal shuttle)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast 2 micron, ori/REP1/REP2/REP3
FT                   3. Schizosaccharomyces pombe, POT1 gene
FT                   -> pMPOT2"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;