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ID   PMTCHI4426 preliminary; circular DNA; SYN; 12500 BP.
AC   ATCC39395;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pMTchi4/4269 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   MTX4 from pBR322 & yeast 2 micron & LEU2 gene
RC   pMTchi4/4269 from MTX4 & pTiA6 & linker
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,670,399 dated June 2, 1987.
CC   A 480 bp HindIII/Sau3AI fragment from pTiA6 was isolated, the Sau3AI
CC   site changed to BamHI, and the ends filled.  HindIII linkers were
CC   added and it was cloned into the HindIII site of MTX4 (ATCC 39276).
CC   An episomal Saccharomyces cerevisiae/E.coli shuttle vector.
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--12.5; PvuII--10.5, 2.45; BamHI--12.5. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMTchi4/4269)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli LE392)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. yeast 2 micron 6318bp
FT                   3. yeast 1400bp, LEU2 gene
FT                   -> MTX4 12000bp
FT                   1. MTX4 HindIII 12000bp
FT                   2. pTiA6 HindIII-Sau3AI 480bp
FT                   BamHI linker 6bp ggatcc
FT                   fill in:fill in
FT                   HindIII linker 6bp aagctt:HindIII linker 6bp aagctt
FT                   -> pMTchi4/4269 12500bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO E. coli Ti gene"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;