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ID   PMTCHI5426 preliminary; circular DNA; SYN; 10800 BP.
AC   ATCC39394;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pMTchi5/4269C - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   MTX5 from pBR322 & yeast 2 micron & LEU2 gene
RC   pMTchi5/4269C from MTX5 & pTiA6 & linker
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,670,399 dated June 2, 1987.
CC   This vector contains the following sites separated by (kb): BamHI -
CC   0.15 - PvuII - 0.33 - BamHI - 9.2 - PvuII - 1.6 - BamHI.
CC   A 480 bp HindIII/Sau3AI fragment from pTiA6 was isolated, the Sau3AI
CC   site changed to BamHI and the ends filled.  BamHI linkers were added
CC   and it was cloned into the BamHI site of MTX5.
CC   An episomal Saccharomyces cerevisiae/E.coli shuttle vector.
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--11.3; PvuII--9.6, 2.0; BamHI--11.3. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMTchi5/4269C)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli LE392)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. yeast 2 micron 6318bp
FT                   3. yeast 1400bp, LEU2 gene
FT                   -> MTX5 12000bp
FT                   1. MTX5 HindIII 12000bp
FT                   2. pTiA6 HindIII-Sau3AI 480bp
FT                   BamHI linker 6bp ggatcc
FT                   fill in:fill in
FT                   HindIII linker 6bp aagctt:HindIII linker 6bp aagctt
FT                   -> pMTchi5/4269C 10800bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO E. coli Ti gene"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   CDS             0..0
FT                   /note="ANT yeast DHFR gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;