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ID   PNC161     preliminary; circular DNA; SYN; 5555 BP.
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AC   M55339;
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DT   01-FEB-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pNC161 - incomplete, origin.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-57
RC   pNC160 from pUC119 & pYe(CEN3)30
RC   pNC161 from pUC118 & pYe(CEN3)30
RA   Rhodes N., Company M., Errede B.;
RT   "A yeast-Escherichia coli shuttle vector containing the M13 origin
RT   of replication";
RL   Plasmid 23:159-162(1990).
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CC   NCBI gi: 209101
CC   NM (pNC161)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(yeast)(Saccharomyces)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE ()
CC   PA (E.coli)(yeast)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC118 3162bp
FT                   2. pYe(CEN3)30 2400bp, CEN3
FT                   -> pNC161 5555bp"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
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SQ   Sequence 57 BP; 12 A; 16 C; 17 G; 12 T; 0 other;
     gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagctt
//