ID   PNF1       preliminary; circular DNA; SYN; 1 BP.
XX
AC   M29400; M29401; M29402;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   E. coli plasmid vector pNF1 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCB532 from Rhodobacter capsulatus nifHDK
RC   pNBR from pBR322
RC   M13::nif from M13mp19 & R.capsulatus nifHDK operon
RC   pNF1 from M13::nif & pNBR
RC   pNF2 from pNF1 & pHP45omega
RC   pNF3 from pNF2 & kan gene
RC   pNF:Q-Zomega from pNF1 & pCB532
RA   Pollock D., Bauer C.E., Scolnik P.A.;
RT   "Transcription of the Rhodobacter capsulatus nifHDK operon is
RT   modulated by the nitrogen source. Construction of plasmid expression
RT   vectors based on the nifHDK promoter";
RL   Gene 65:269-275(1988).
XX
RN   [2]
RC   pDPT42 from pDPT31 & pBR322
RC   pDPT44 from pDPT31 & pBR322
RC   pDPT51 from R751 & RSF2011
RC   pDPT55 from R751 & RSF2233
RC   pRPSB4 from pDPT42 & pRPS404
RC   pRPSB5 from pDPT42 & pRPS404
RC   pRPSB9 from pDPT42 & pRPS404
RC   pRPSB10 from pDPT42 & pRPS404
RC   pRPSB31 from pDPT42 & pRPS404
RC   pRPSB104 from pDPT42 & pRPS404
RC   pRPSB105 from pDPT42 & pRPS404
RC   pRPSB108 from pDPT42 & pRPS404
RC   pRPSB40 from pDPT42 & pRPS404
RC   pRPSE30 from pDPT44 & pRPS404
RC   pRPSE2 from pDPT44 & pRPS404
RC   pRPSEB1 from pBR322 & pRPS404
RC   pRPSEB2 from pRPSEB1 & pRPS404
RA   Taylor D.P., Cohen S.N., Clark W.G., Marrs B.L.;
RT   "Alignment of the genetic and restriction maps of the photosynthetic
RT   region of the Rhodopseudomonas capsulata chromosome by a
RT   conjugation-mediated marker rescue technique";
RL   J. Bacteriol. 154:580-590(1983).
XX
RN   [3]
RC   pDPT31 from pSC301
RC   pDPT270 from NR1 & cat gene
RA   Taylor D.P., Cohen S.N.;
RT   "Structural and functional analysis of cloned DNA segments containing
RT   the replication and incompatibility regions of a miniplasmid derived
RT   from a copy number mutant of NR1";
RL   J. Bacteriol. 137:92-104(1979).
XX
RN   [4]
RC   pBLM2 from RP1
RC   pRPS404 from Rhodopseudomonas
RA   Marrs B.L.;
RT   "Mobilization of th egenes for photosynthesis from Rhodopseudomonas
RT   capsulata by a promiscuous plasmid";
RL   J. Bacteriol. 146:1003-1012(1981).
XX
RN   [5]
RC   RSF2011 from ColE1 & Tn3
RC   RSF2233 from ColE1 & Tn3
RA   Rodriguez R.L., West W.R., Heyneker H.L., Bolivar F., Boyer H.W.;
RT   "Characterizing wild-type and mutant promoters of the tetracycline
RT   resistance gene in pBR313";
RL   Nucleic Acids Res. 6:3262-3287(1979).
XX
RN   [6]
RC   pKP772 from pBR322
RA   Peden K.;
RT   ;
RL   Unpublished (1985).
XX
CC   nifHDK promoter. MCS. in GenBank.
CC   NM (pNF1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC4-KIXX)(pCB532)
CC   BR (pNF2)(pNF3)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 NdeI
FT                   fill in
FT                   -> pNBR
FT                   1. Tn5
FT                   transposition
FT                   2. R.capsulatus HindIII-HindIII 11800bp, nifHDK operon
FT                   -> nifHDK-Tn5-nifHDK
FT                   1. M13mp19 remove small HindIII-SalI, MCS
FT                   2. nifHDK-Tn5-nifHDK HindIII-SalI 2200bp, Tn5-nifH
FT                   -> M13::nif
FT                   1. M13::nif cacatg 322..322, nif promoter
FT                   mutagenesis to catatg NdeI
FT                   HindIII-BamHI, Tn5-MCS
FT                   2. pNBR remove small HindIII-BamHI
FT                   -> pNF1"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 381 BP; 88 A; 119 C; 98 G; 76 T; 0 other;
     agttcatccc gcgcgatgaa gacgtgatgg tgcatttcac cggcaccccc ggcggctcgg
     tctgaaaccg ccgcaaggac cggaaaacgc cgcgctggcg gggtttttca tttcctcccg
     acagagggcc cgacaaaacc ccgcccgaca ccgcaggccc tacaatttgt cggccttgtt
     ccatttcgaa caaaaccttc aacaccatga tttcgcgtca ttatttgcga aaattccggt
     tggcacgatg gctgctgtag aagctgtgag cccggttagg aaccgtctcg atattcgtga
     agcaccaacc ccaaggagcc accacatggg caaactccgt cagatcgcct tctacggcaa
     aggtggtatc ggcaagtcga c
//