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ID   PNF3Y      preliminary; circular DNA; SYN; 7000 BP.
AC   ATCC37188;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pNF3 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pYE24 from yeast 2 micron
RC   pNF1 from pYE24 & pMC874
RC   pNF2 from pNF1
RC   pNF3 from pNF2
RC   pNF4 from pNF3
RC   pNF1000 from pNF1 & yeast DNA
RC   pNF2000 from pNF2 & yeast DNA
RC   pNF3000 from pNF3 & yeast DNA
RA   Naumovski L., Friedberg E.C.;
RT   "Construction of plasmid vectors that facilitate subcloning and
RT   recovery of yeast and Escherichia coli DNA fragments";
RL   Gene 22:203-209(1983).
CC   SalI sites flank the BamHI cloning site for ready removal of the
CC   insert. [1]
CC   This plasmid is identical to pNF2 (ATCC 37187) except for the deletion
CC   of the 2.2 kb EcoRI fragment containing the yeast 2 micron origin of
CC   replication. [1]
CC   A integration shuttle vector for subcloning and recovery of DNA from
CC   Saccharomyces cerevisiae. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pNF3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pNF2 remove EcoRI-EcoRI 2240bp 2..2242,
FT                   \ YEp24/7800bp
FT                   -> pNF3 7800bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT Saccharomyces G418 resistance gene (G418)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;