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ID   PNF4       preliminary; circular DNA; SYN; 1 BP.
AC   ATCC37189;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pNF4 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pYE24 from yeast 2 micron
RC   pNF1 from pYE24 & pMC874
RC   pNF2 from pNF1
RC   pNF3 from pNF2
RC   pNF4 from pNF3
RC   pNF1000 from pNF1 & yeast DNA
RC   pNF2000 from pNF2 & yeast DNA
RC   pNF3000 from pNF3 & yeast DNA
RA   Naumovski L., Friedberg E.C.;
RT   "Construction of plasmid vectors that facilitate subcloning and
RT   recovery of yeast and Escherichia coli DNA fragments";
RL   Gene 22:203-209(1983).
CC   A pBR322 derivative containing a kanamycin resistance gene and a
CC   single BamHI site flanked immediately on each side by SalI sites.
CC   Vector that facilitates subcloning and recovery of inserts.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pNF4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pNF3 remove HindIII-HindIII 1166bp
FT                   \ 2273..3439, YEp24 URA3/6700bp
FT                   -> pNF4 6700bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;