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ID   PNRC747    preliminary; circular DNA; SYN; 1 BP.
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AC   IG8028;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pNRC747 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pNRC747 from pMC1403 & oligo
RC   pNRC747-10 from pNRC747
RC   pNRC747-14 from pNRC747
RC   pNRC747-15 from pNRC747-14
RC   pNRC747-11 from pNRC747-10 & linker
RC   from pNRC747-11 & pBR322
RC   pNRC747-lambdap111 from pNRC747-10 & lambda cI857 Sam7
RA   Thomas D.Y., Dubuc G., Narang S.;
RT   "Escherichia coli plasmid vectors containing synthetic translational
RT   initiation sequences and ribosome binding sites fused with the lacZ
RT   gene";
RL   Gene 19:211-219(1982).
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CC   NM (pNRC747)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN ()
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMC1403 SmaI, in lacZ gene
FT                   2. oligo ATG-EcoRI-ATG 12bp catgaattcatg
FT                   -> pNRC747"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-ATG-EcoRI-ATG-BamHI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 12 BP; 4 A; 2 C; 2 G; 4 T; 0 other;
     catgaattca tg
//