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ID   POG44      preliminary; circular DNA; SYN; 1 BP.
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AC   IG2097; X52327;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Mammal/E. coli phagemid vector pOG44 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   plasmid from pSKS105 & linker
RC   pFRTbetaGAL from plasmid & pSV2
RC   plasmid2 from pUC19 & FRT gene & pMC1neo-polyA
RC   pNEObetaGAL from pFRTbetaGAL & plasmid2
RC   pOG28 from pCDM8 & mouse DHFR gene
RC   pOG44 from pCDM8 & pMLSIScat & yeast FLP recombinase & yeast 2 micron
RC   pOG45 from pNEObetaGAL & pUC19
RC   pOG45A from pOG45
RA   O'Gorman S., Fox D.T., Wahl G.W.;
RT   "Recombinase-mediated gene activation and site-specific integration
RT   in mammalian cells";
RL   Science 251:1351-1355(1991).
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RN   [2]
RC   pMLSIScat from cat gene
RA   Huang M.T., Gorman C.M.;
RT   "Intervening sequences increase efficiency of RNA 3' processing
RT   and accumulation of cytoplasmic RNA";
RL   Nucleic Acids Res. 18:937-947(1990).
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CC   NM (pOG44)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (coding)
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli)(mammalian tissue culture cells)
CC   CP ()
CC   FN (gene targeting)
CC   SE (color blue/white)
CC   PA (pBluescript II KS+)(CMV)
CC   BR (pOG45)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. SV40 BamHI 5243bp 2534..2534
FT                   -> pJY1 9604bp
FT                   1. pJY1 remove 1393bp, pBR322 1092..2485 poison seq
FT                   \ 8211bp
FT                   -> pJYM 8211bp
FT                   1. pJYM remove BamHI-BamHI 5243bp, SV40
FT                   -> pML 2968bp
FT                   1. pML, SV40 early promoter
FT                   2. adenovirus 186bp, MLP 5' UTR 14 to 200
FT                   3. oligo, IgG variable region splice site
FT                   4. SV40, late polyA
FT                   -> pMLSIS
FT                   1. pMLSIS
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pMLSIScat
FT                   1. pCDM8, CMV early promoter
FT                   2. pMLSIScat, 5' leader/intron
FT                   3. yeast 750bp 5568..6318, FLP recombinase gene
FT                   2. pMLSIScat, SV40 late polyA
FT                   4. yeast 2 micron 626bp, ori
FT                   -> pOG44"
FT   promoter        0..0
FT                   /note="PRO CMV"
FT   misc_feature    0..0
FT                   /note="intron"
FT   CDS             0..0
FT                   /note="GEN S. cerevisiae FLP recombinase gene"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 late gene"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS (21 unique restriction sites)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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