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ID   POG45      preliminary; circular DNA; SYN; 1 BP.
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AC   IG2098; X52327;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Mammal/E. coli phagemid vector pOG45 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   plasmid from pSKS105 & linker
RC   pFRTbetaGAL from plasmid & pSV2
RC   plasmid2 from pUC19 & FRT gene & pMC1neo-polyA
RC   pNEObetaGAL from pFRTbetaGAL & plasmid2
RC   pOG28 from pCDM8 & mouse DHFR gene
RC   pOG44 from pCDM8 & pMLSIScat & yeast FLP recombinase & yeast 2 micron
RC   pOG45 from pNEObetaGAL & pUC19
RC   pOG45A from pOG45
RA   O'Gorman S., Fox D.T., Wahl G.W.;
RT   "Recombinase-mediated gene activation and site-specific integration
RT   in mammalian cells";
RL   Science 251:1351-1355(1991).
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CC   NM (pOG45)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (coding)
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli)(mammalian tissue culture cells)
CC   CP ()
CC   FN (gene targeting)
CC   SE (color blue/white)
CC   PA (pBluescript II KS+)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSKS105 10021bp
FT                   linker
FT                   -> plasmid 10021bp
FT                   1. SV40 StuI-PvuII 323bp 5194..5243..273,
FT                   \ early promoter/HindIII-PvuII 344bp 5172..5243..273
FT                   2. pBR322 3045bp 324..3369, ori/amp gene
FT                   3. SV40 BamHI-MboI 237bp 2534..2771,
FT                   \ small t antigen [3370..3606]
FT                   4. SV40 MboI-? 521bp 2771..3292, polyA [3606..4127]
FT                   -> pSV2 4125bp
FT                   1. plasmid 10021bp
FT                   2. pSV2 4125bp
FT                   -> pFRTbetaGAL
FT                   1. pUC19 2686bp
FT                   2. FRT gene
FT                   3. pMC1neo-polyA, neo gene/polyA
FT                   -> plasmid2
FT                   1. pFRTbetaGAL, FRT gene
FT                   2. plasmid2, neo gene/polyA
FT                   -> pNEObetaGAL
FT                   1. pNEObetaGAL, neo gene/3' FRT
FT                   2. pUC19 2686bp, MCS
FT                   -> pOG45"
FT   misc_binding    0..0
FT                   /note="MCS KpnI-HincII-ClaI-HindIII"
FT   CDS             0..0
FT                   /note="GEN S. cerevisiae FLP recombination targets
FT                   (FRT)"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo);
FT                   kanamycin resistance gene (kan);
FT                   G418 resistance gene (G418)"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-SpeI-NotI-SacI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS (21 unique restriction sites)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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