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ID   POJ31      preliminary; circular DNA; SYN; 5000 BP.
AC   ATCC77416;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Actinomyces/E.coli cosmid vector pOJ31 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pHSS6deltaH from pHSS6
RC   pHSSLys from pHSS6deltaH & p1-L13, LYS2 gene
RC   p18Lys from pHSSLys & pTZ18R
RC   pICL from p18Lys & linker
RC   pHSSLysL from pHSSLys & T7 linker
RC   plasmid from pBluescript II & linker & pYAC4, URA
RC   pLUS from plasmid & pHSSLysL
RC   [pOJ31 from pBR322 & kan gene & lambda, cos]
RA   Hermanson G.G., Hoekstra M.F., McElligott D.L., Evans G.A.;
RT   "Rescue of end fragments of yeast artificial chromosomes by
RT   homologous recombination in yeast";
RL   Nucleic Acids Res. 19:4943-4948(1991).
CC   Medium copy number shuttle vector allowing lacZalpha
CC   complementation. Constructed from the cosmid vector pFD304 by addition
CC   of a lacZalpha-polylinker fragment and a 2.4 kb PstI-SalI fragment
CC   containing the JHJ-3 replicon. The JHJ-3 replicon permits replication
CC   in the following actinomycete genera:  Streptomyces, Saccharopolyspora
CC   and Amycolatopsis.
CC   The order of the major features in the plasmid is: JHJ-3
CC   ori - neo - ClaI* - synthetic promoter - pMB1 ori - cos site - lac
CC   promoter - SpeI/MCS/XbaI - lacZ.
CC   The ClaI* site is methylated, making
CC   the ClaI site in the polylinker unique in E. coli dam+ strains. [2]
CC   Growth medium: LB plus kanamycin (50 ug/ml)(ATCC number 1236) 37C
CC   Deposited by: C.V.Dery
CC   NM (pOJ31)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli)(actinomycetes)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. kan gene
FT                   3. lambda, cos
FT                   -> pOJ31 5000bp"
FT   promoter        0..0
FT                   /note="PRO lac"
FT   promoter        0..0
FT                   /note="PRO T7"
FT   misc_binding    0..0
FT                   /note="MCS SpeI-XbaI-NotI-EcoRV-BamHI-NdeI-SalI-PstI-
FT                   EcoRI-SacI-SmaI-SphI-NsiI-ClaI-BglII-XhoI-StuI-SpeI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI actinomycetes JHJ-3"
FT   CDS             0..0
FT                   /note="ANT E. coli kan gene"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ' gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;