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ID   POK12      preliminary; circular DNA; SYN; 144 BP.
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AC   M74307;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pOK12 - incomplete, MCS.
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KW   cloning vector; MCS.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-144
RC   MCS from pMTL23 & pBluescript SK
RC   pUC21 from pUC118 & linker MCS
RC   pUK21 from pUC21 & Tn903
RC   pUC6S from MCS & pUC6; [pUC with no lacZ]
RC   pOK12 from pUC21 & Tn903 & pACYC177
RA   Vieira J., Messing J.;
RT   "New pUC-derived cloning vectors with different selectable markets
RT   and DNA replication origins";
RL   Gene 100:189-194(1991).
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CC   NCBI gi: 209215
CC   NM (pOK12)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC21)(Tn903)(pACYC177)
CC   BR (pUK21)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove BamHI-PvuII 1691bp 376..2067,
FT                   \ tet/2670bp
FT                   Klenow:
FT                   -> pMTL1 2670bp [unique BamHI]
FT                   1. pMTL1 remove HaeII-HaeII 622bp, rop/nic
FT                   \ pBR322 1730..2352/2048bp
FT                   -> pMTL2 2048bp [no NdeI at 2297]
FT                   1. pMTL2 remove TaqI-TaqI 1444bp, PstI/HincII in amp
FT                   \ pBR322 2574..4018
FT                   2. pUC8 TaqI-TaqI 1444bp 998..2442, amp gene
FT                   -> pMTL3 2048bp
FT                   1. pMTL3 AatII-RsaI 238bp 4286..164
FT                   T4 DNA polymerase:
FT                   -> pMTL4 1810bp
FT                   1. M13mp19 remove SphI-SalI 8bp 6245..6253, MCS/7241bp
FT                   2. oligo StuI-AatII 29bp
FT                   \ ggcctcgagatctccatggacgcgtgacg
FT                   -> M13mt121 7270bp
FT                   1. pMTL4 HaeII 1810bp 0..0
FT                   2. M13mt121 HaeII-HaeII 540bp, lacZ gene
FT                   3. oligo 80bp
FT                   -> pMTL23 2500bp
FT                   1. pMTL23, MCS
FT                   2. pBluescript SK+, MCS/T7
FT                   -> MCS linker
FT                   1. pUC21 HaeII-HaeII 540bp, lacZ gene/M13mp19
FT                   2. pACYC177 HaeII-HaeII 1430bp 1596..3026, Tn903 kan
FT                   3. pACYC177, p15A ori
FT                   4. MCS linker
FT                   -> pOK12"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 144 BP; 32 A; 41 C; 39 G; 32 T; 0 other;
     atgaccatga ttacgccact agtccgaggc ctcgagatct atcgatgcat gccatggtac
     ccgggagctc gaattcgaag cttctgcaga cgcgtcgacg tcatatggat ccgatatcgc
     cgtggcggcc gctctagaac tagt
//