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ID   POPI3CAT   preliminary; circular DNA; SYN; 1 BP.
AC   IG2072;
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Mammal/E. coli phagemid vector pOPI3 CAT - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   p3'ss from pInt.lacI & PyF9-1CAT & lambda, cos
RC   pOPI3 CAT from cat gene
RC   pOPRSVI CAT from cat gene
RA   DuCoeur L.C., Wyborski D.L., Short J.M.;
RT   ;
RL   Strategies 5:70-72(1992).
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli)(mammal cell line)
CC   CP ()
CC   FN (expression eukaryote)(expression inducible)
CC   SE ()
CC   PA (pBR322)(f1)(RSV)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. RSV, LTR
FT                   3. E. coli, lac promoter
FT                   4. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   5. SV40, tk gene
FT                   6. G418 gene
FT                   7. f1, ori
FT                   -> pOPI3 CAT"
FT   misc_binding    0..0
FT                   /note="SIT BstXI"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_feature    0..0
FT                   /note="TATA"
FT   misc_feature    0..0
FT                   /note="TSP"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   intron          0..0
FT                   /note="SIT E. coli lac repressor binding sites; three"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene operator,
FT                   that binds lac repressor"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 thymidine kinase (TK) gene"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo);
FT                   kanamycin resistance gene (kan);
FT                   G418 resistance gene (G418)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1+"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;