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ID   PPBAC      preliminary; circular DNA; SYN; 1 BP.
XX
AC   IG2091;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Insect/E. coli phagemid vector pPbac - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJVP10Z from pUC series
RC   pMbac from pJVP10Z
RC   pPbac from pJVP10Z
RA   Lernhardt W.;
RT   ;
RL   Strategies 6:20-21(1993).
XX
CC   NM (pPbac)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli)(insect cell line SF9)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE (color blue/white)
CC   PA (pJVP10Z)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18, ori/amp/lacZ/f1 ori
FT                   -> pJVP10Z
FT                   1. pJVP10Z, ori/amp/lacZ/f1 ori
FT                   2. human placenta, alkaline phosphatase secretory sig
FT                   3. AcMNPV, p10 promoter/polyhedrin gene
FT                   -> pPbac"
FT   misc_feature    0..0
FT                   /note="baculovirus sequences for recombination"
FT   polyA_signal    0..0
FT                   /note="PLA AcMNPV polyhedrin gene"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   misc_feature    0..0
FT                   /note="human placental alkaline phosphatase secretory
FT                   signal sequence"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_feature    0..0
FT                   /note="stuffer fragment"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   promoter        0..0
FT                   /note="PRO AcMNPV p10 gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   polyA_signal    0..0
FT                   /note="PLA E. coli lac gene"
FT   misc_feature    0..0
FT                   /note="baculovirus sequences for recombination"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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