ID   PPCPHI     preliminary; circular DNA; SYN; 1 BP.
XX
AC   IG8045;
XX
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pPCphi - incomplete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pPC21, pPC22, pPC23, pPC24 from pBR322 & linker & pOP203, lacUV5
RC   pPC31, pPC32, pPC33 from pBR322 & linker & pOP203, lacUV5
RC   [lambda plac5-1 from lambda plac5]
RC   lambda deltaZ1 from lambda plac5-1 & lacUV5 region
RC   lambda deltaZ2 from lambda plac5-1 & lacUV5 region
RC   lambda deltaZ3 from lambda plac5-1 & lacUV5 region
RC   pPCphi1 from pBR322 & lambda deltaZ1
RC   pPCphi2 from pBR322 & lambda deltaZ2
RC   pPCphi3 from pBR322 & lambda deltaZ3
RA   Charnay P., Perricaudet M., Galibert F., Tiollais P.;
RT   "Bacteriophage lambda and plasmid vectors, allowing fusion of
RT   cloned genes in each of the three translational phases";
RL   Nucleic Acids Res. 5:4479-4494(1978).
XX
CC   NM (pPCphi)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda plac5-1 EcoRI
FT                   2. E. coli EcoRI-EcoRI 205bp, lacUV5 region
FT                   S1 nuclease
FT                   EcoRI linker 8bp ggaattcc:EcoRI linker 8bp ggaattcc
FT                   EcoRI-EcoRI 200bp, lacUV5 region
FT                   -> phage
FT                   1. phage
FT                   recombination, to keep 1 lacUV5 and remove lacZ
FT                   -> lambda deltaZ1
FT                   1. pBR322 remove EcoRI-HindIII 31bp 4360..4361..30,
FT                   \ 4330bp
FT                   2. lambda deltaZ1 EcoRI-HindIII 500bp, lacUV5 region
FT                   -> pPCphi1 4800bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//