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ID   PPI251     preliminary; circular DNA; SYN; 8800 BP.
XX
AC   X06779; V01520; X69493; ATCC37236;
XX
DT   30-SEP-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Drosophila/E.coli plasmid vector ppi25.1 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4734
RC   ppi25.1
RA   O'Hare K.;
RT   ;
RL   Submitted (09-FEB-1988) on tape to the EMBL Data Library by:
RL   O'Hare K., Department Biochemistry, Imperial College, London, UK.
XX
RN   [2]
RC   ppi25.1, ppi25 from pBR322 & P element
RC   pS25.1 from pBR322 & Drosophila DNA
RC   [ppi25.7 from pBR322 & Drosophila DNA]
RA   Spradling A.C., Rubin G.M.;
RT   "Transposition of cloned P elements into Drosophila germ line
RT   chromosomes";
RL   Science 218:341-347(1982).
XX
RN   [3]
RC   pry1, pry3 from p6.1 & rosy gene
RC   pDm2844S8.5 from pry1
RC   pm12.8 from pry1
RC   pch1 from p6.1 & Drosophila chorion gene/66D region
RA   Rubin G.M., Spradling A.C.;
RT   "Genetic transformation of Drosophila with transposable element
RT   vectors";
RL   Science 218:348-353(1982).
XX
RN   [4]
RP   1-4734
RC   lambda pi12, lambda pi25 from Charon 28 & Drosophila P pi2
RC   lambda pi30, lambda pi44 from Charon 28 & Drosophila P pi2
RC   lambda S12, lambda S25 from Charon 28 & Drosophila M strain
RC   lambda S30, lambda S44 from Charon 28 & Drosophila M strain
RC   ppi25.1 from lambda pi25 & pBR322
RC   ppi12.20 from lambda pi12 & pBR322
RC   pS25.1 from lambda S25 & pBR322
RC   pS12.20 from lambda S12 & pBR322
RC   p6.1 from lambda & pBR322 & Drosophila white locus
RA   O'Hare K., Rubin G.M.;
RT   "Structures of P transposable elements and their sites of
RT   insertion and excision in the Drosophila melanogaster genome";
RL   Cell 34:25-35(1983).
XX
RN   [5]
RC   lambda m1.1, lambda m1.2 from Drosophila white locus
RC   lambda pi.3, lambda pi.10 from Charon 28 & Drosophila white locus
RC   lambda pi40.8 from Charon 28 & Drosophila white locus
RC   lambda 6.1, lambda 6.4 from pBR322 & Drosophila white locus
RC   lambda 12.3, lambda 12.4, lambda 12.6 from pBR322 & white locus
RC   lambda 12.12 from pBR322 & white locus
RC   p6.1 from pBR322 & lambda 6.1
RA   Rubin G.M., Kidwell M.G., Bingham P.M.;
RT   "The molecular basis of P-M hybrid dysgenesis: the nature of
RT   induced mutations";
RL   Cell 29:987-994(1982).
XX
RN   [6]
RC   lambda 103 from lambda & Drosophila chorion gene/66D region
RC   lambda 104 from lambda & Drosophila chorion gene/66D region
RC   lambda 302 from lambda & Drosophila chorion gene/66D region
RC   p103.48, p103.29 from lambda 103, Drosophila chorion gene/66D region
RC   p104.60, p104.41 from lambda 104, Drosophila chorion gene/66D region
RC   p302.77, p302.42 from lambda 302, Drosophila chorion gene/66D region
RA   Spradling A.C.;
RT   "The organization and amplification of two chromosomal domains
RT   containing Drosophila chorion genes";
RL   Cell 27:193-201(1981).
XX
CC   The sequence is from cytological location 17C of strain
CC   pi2 of Drosophila melanogaster.
CC   Plasmid used for P-element mediated gene transfer.
CC   Contains the complete 2.9 kb P element of Drosophila melanogaster.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (ppi25.1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (D.melanogaster)(Drosophila)(E.coli K-12)(E.coli HB101)(E.coli)
CC   CP ()
CC   FN (gene transfer)(transformation)(transposable element)
CC   SE ()
CC   PA (Drosophila strain pi2 location 17C)(P element)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Charon 28
FT                   2. Drosophila P pi2
FT                   -> lambda pi25
FT                   1. pBR322 BamHI 4361bp 376..376
FT                   2. lambda pi25 BamHI-BamHI 2900bp, P element
FT                   -> ppi25.1 8800bp"
FT   cellular        1..995
FT                   /note="from Drosophila strain pi2, location 17C"
FT   misc_feature    812..812
FT                   /note="A in Canton S"
FT   misc_feature    902..902
FT                   /note="A in Canton S"
FT   misc_feature    938..938
FT                   /note="T in Canton S"
FT   repeat_region   988..995
FT                   /note="RPT direct repeat 1"
FT   transposon      996..3902
FT                   /note="TRN Drosophila P element"
FT   repeat_region   3903..3910
FT                   /note="RPT direct repeat 1"
FT   cellular        3911..4734
FT                   /note="from Drosophila strain pi2, location 17C"
FT   misc_feature    3996..3996
FT                   /note="ADP AAAAAAAG inserted in Canton S"
FT   misc_feature    4092..4092
FT                   /note="G in Canton S"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 4734 BP; 1501 A; 878 C; 909 G; 1446 T; 0 other;
     ggatcccaat ccgcatcgaa taagacgcaa acttttgcat cgtatattga aaagatgcgc
     tatctccatg gcaaagtttt tgcggtcgtc gcagacaaaa gacgcgtgtt cccttttaaa
     gtttcattaa tcaatggctc gggtggtgga tgtggatgtg gctgtagcta ttgatgtagc
     tggaggggtt ctgccacagc tccggctata gcttttatta tttcaattgc ggaacggtgc
     ccggggctca aaaatatgag gcatcattta tcaggccgcc attccaccac tccactacgt
     cataccattc cgttctggtg cggattggct gtctgggctg tggatttcaa attggcagca
     acaatgccgt gattaatggc cgtcaaagcg aaagagtcaa caaatgtcaa gcgacaaact
     gcaattggca attggaggga gggggggtgg tggtggtggt aaagaggacc aaatcccaaa
     aaaccccata ccataacata taccatatac catactatac catggtagga atcccttggc
     ccgcgatgaa atgaatgcca acacaagtgc gggttaattt tttcaaatgt ttgttaatta
     catttattgt ttccttttgt ttttgcattt gcatttgttt ttatgttttt ttttctctcg
     tttctcttta gttttgattt ctttcacttg caatcgttct ggctgctttc attattactg
     attgccggcc aaaactcgga ttatggtttg tttttgtaat gtatatatat gcacgtatat
     ataagtctaa gcgggtttat cttacacttt agatattttt gatttaactt tatgcctgct
     ctctaacaat acaatacaaa aaaaatattg gataatcgct cttacttatt tccgattatt
     agatattatt acgatattta acatagtttc ggtaattcga tagataggta gtattgttta
     tcgctcgtag atgatagact gttgcttata cacaccatga tgaaataaca taaggtggtc
     ccgtcgaaag ccgaagctta ccgaagtata cacttaaatt cagtgcacgt ttgcttgttg
     agaggaaagg ttgtgtgcgg acgaattttt ttttgaaaac attaaccctt acgtggaata
     aaaaaaaatg aaatattgca aattttgctg caaagctgtg actggagtaa aattaattca
     cgtgccgaag tgtgctatta agagaaaatt gtgggagcag agccttgggt gcagccttgg
     tgaaaactcc caaatttgtg atacccactt taatgattcg cagtggaagg ctgcacctgc
     aaaaggtcag acatttaaaa ggaggcgact caacgcagat gccgtaccta gtaaagtgat
     agagcctgaa ccagaaaaga taaaagaagg ctataccagt gggagtacac aaacagagta
     agtttgaata gtaaaaaaaa tcatttatgt aaacaataac gtgactgtgc gttaggtcct
     gttcattgtt taatgaaaat aagagcttga gggaaaaaat tcgtactttg gagtacgaaa
     tgcgtcgttt agagcagcag ctgagggagt ctcaacagtt ggaggagtct ctacgcaaaa
     tcttcacgga cacgcagata cggatactga agaatggtgg acaaagagct acgttcaatt
     ccgacgacat ttctacagct atttgtctcc acaccgcagg ccctcgagcg tataaccatc
     tgtacaaaaa aggatttcct ttgcccagtc gtacgacttt gtacagatgg ttatcagatg
     tggacataaa aagaggatgt ttggatgtgg tcatagacct aatggacagt gatggagttg
     atgacgccga caagctttgc gtactcgctt tcgacgagat gaaggtcgct gctgccttcg
     agtatgacag ctctgctgat attgtttacg agccaagcga ctatgtccaa ctggctattg
     ttcgtggtct aaaaaaatcg tggaagcagc cagttttttt cgattttaat acccgaatgg
     acccggatac tcttaacaat atattaagga aactgcatag gaaaggatat ttagtagttg
     ctattgtatc cgatttaggt accggaaacc aaaagctatg gacagagctc ggtatatcag
     aatgtaagtt tcgtatatta caaaaatcag ataatccttg aaattccatt ttttagcaaa
     aacctggttt agccatcctg cagatgacca tttaaagatt ttcgtttttt cggatacgcc
     acatttaatt aagttagtcc gtaaccacta tgtggattcc ggattaacaa taaatgggaa
     aaaattaaca aaaaaaacaa ttcaggaggc acttcatctt tgcaacaagt ccgatctgtc
     tatcctcttt aaaattaatg aaaatcacat taatgttcga tcgctcgcaa aacagaaggt
     taaattggct acccagctgt tttcgaatac caccgctagc tcgatcagac gctgctattc
     attggggtat gacattgaaa atgccaccga aactgcggac ttcttcaaat tgatgaatga
     ttggttcgac atttttaatt ctaaattgtc cacatccaat tgcattgagt gctcgcaacc
     ttatggcaag cagttggata tacagaatga tattttgaat cgaatgtcgg aaattatgcg
     aacaggaatt ctggataaac ccaaaaggct cccatttcaa aaaggtatca ttgtgaataa
     tgcttcgctt gatggcttgt ataaatattt gcaagaaaac ttcagtatgc aatacatatt
     aacaagccgt ctcaaccaag acattgtgga gcattttttt ggcagcatgc gatcgagagg
     tggacaattc gaccatccca ctccactgca gtttaagtat aggttaagaa aatatataat
     aggtatgaca aatttaaaag aatgcgtaaa caaaaatgta attccatgat ttataattgt
     ttaatgttta gctatatgtt tcaggaaagt ttcagttgag aatgtaggta gttatgtgct
     gtctattgtg ttttgtcttt tatctgtttc ttttcatttt attatttaat cattatcctt
     ttgcttatcc agccaggaat acagaaatgt taagaaattc gggaaatatc gaagaggaca
     actctgaaag ctggcttaat ttagatttca gttctaaaga aaacgaaaat aaaagtaaag
     atgatgagcc tgtcgatgat gagcctgtcg atgagatgtt aagcaatata gatttcaccg
     aaatggatga gttgacggag gatgcgatgg aatatatcgc gggctatgtc attaaaaaat
     tgagaatcag tgacaaagta aaagaaaatt tgacatttac atacgtcgac gaggtgtctc
     acggcggact tattaagccg tccgaaaaat ttcaagagaa gttaaaagag ctagaatgta
     tttttttgca ttatacaaat aataataatt ttgaaattac aaataatgta aaggaaaaat
     taatattagc agcgcgaaac gtcgatgttg ataaacaagt aaaatctttt tattttaaaa
     ttagaatata ttttagaatt aagtacttca acaaaaaaat tgaaattaaa aatcaaaaac
     aaaagttaat tggaaactcc aaattattaa aaataaaact ttaaaaataa tttcgtctaa
     ttaatattat gagttaattc aaaccccacg gacatgctaa gggttaatca acaatcatat
     cgctgtctca ctcagactca atacgacact cagaatacta ttcctttcac tcgcacttat
     tgcaagcata cgttaagtgg atgtctcttg ccgacgggac caccttatgt tatttcatca
     tgatacacac actttggttt ctcccgtact tagtatattc gctagctcct ttcgaacgct
     aattacgcat attgattagt gttacattag attgcaaaaa aaaaaaggat taataaatac
     atccatattg taactagttc gcttggttat agatatacac acgcacgcac acaaatcctt
     aactttacat ccacctatct taggcgcttt acatctaacg cgctggatca tcgtggatct
     ggccatcata cctcatctac aatccatcta gaaatcatcc agctccagct acttctatac
     aacgttcgcc gtggaaagaa cattctttag cctcgcacta aactaaactt aaataataat
     caacaacgtt gctccaacag acttaaaatg gaattagaac aaaaatcatg tgtgcttaag
     tcgtgtgcag aatgtctcaa tatttacaca tcaaagttca cttttgccaa ttgccctcta
     gctcctctag ctcctcatca tctatctctc ttcatctgac tctactctac gatctgcggc
     tcttcggatt cgaaggacac atcgaaaatc gaacagtgat cggtggggat gtttagctct
     ctcgatttcg cagtccacaa aatccgattc tctccaccac cagccgccct tgtcattcat
     tccacattca tcagtttagt ccttcgttta tttttttttt tttaactttt tggcactgct
     ttgaatttct tttggcaccg cacgcttcga gcgtgaaaat aaaattattt atgcatatac
     atagataaat acgaatatat ctatatggag tatgtaaaaa aaaaaaaagg atcc
//