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ID   PPI257     preliminary; circular DNA; SYN; 9000 BP.
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AC   M30841; ATCC37237;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Drosophila/E.coli plasmid vector ppi25.7 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   ppi25.1, ppi25 from pBR322 & P element
RC   pS25.1 from pBR322 & Drosophila DNA
RC   [ppi25.7 from pBR322 & Drosophila DNA]
RA   Spradling A.C., Rubin G.M.;
RT   "Transposition of cloned P elements into Drosophila germ line
RT   chromosomes";
RL   Science 218:341-347(1982).
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CC   This vector contains the complete 2.9 kb P element of Drosophila
CC   melanogaster.
CC   A helper plasmid for P-mediated gene transfer which provides
CC   transposase function.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (ppi25.7)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(Drosophila melanogaster)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Charon 28
FT                   2. Drosophila P pi2
FT                   -> lambda pi25
FT                   1. pBR322 BamHI 4361bp 376..376
FT                   2. lambda pi25 BamHI-BamHI 2900bp, P element
FT                   -> ppi25.7 9000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI-SacI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//