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ID   PPOP10     preliminary; circular DNA; SYN; 1 BP.
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AC   IG1099; X03453; M22642; M19164; M19165; M19166; M19167; M19168;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pPop10 [tm] - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pZD1, pZD2 from pZIPneoSV(x)1 & oligo
RC   lambda ZD31 from pZD1 & Charon 30
RC   lambda ZD32 from pZD2 & Charon 30
RC   rpZD1 from lambda ZD31
RC   rpZD2 from lambda ZD32
RC   rpZDD1 from rpZD1
RC   rpZDD2 from rpZD2
RC   rpZd5 from rpZD1
RC   lambda ZD5 from rpZd5 & lambda ZD31
RC   p3P0 from pZIPneoSV(x)1 & MMLV
RA   Murphy A.J., Efstratiadis A.;
RT   "Cloning vectors for expression of cDNA libraries in mammalian cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:8277-8281(1987).
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CC   NM (pPop10 [tm])
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (Invitrogen)
CC   HO (E.coli 293-EBNA)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (EBV)(lambda)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cI gene
FT                   2. EBV, EBNA-1
FT                   3. SV40, tk gene
FT                   4. RSV, LTR
FT                   5. E. coli 1142bp, hyg gene
FT                   -> pPop10"
FT   promoter        0..0
FT                   /note="PRO cytomegalovirus (CMV)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA mammal thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   promoter        complement(0..0)
FT                   /note="PRO mammal thymidine kinase (TK) gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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