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ID   PPW262     preliminary; circular DNA; SYN; 7800 BP.
AC   IG7029;
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pPW262 - incomplete.
KW   cloning vector; insertion sequence.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pPW2 from pBR327 & pWM8
RC   pPW26 from pPW2
RC   pMLP2 from pML24 & pHC624
RC   pMLP21 from pMLP2 & pUN121, cI857
RC   pPW262 from pPW26 & pMLP21
RC   pPW263 from pPW262
RC   pPW121 from pUN121
RA   Puta F., Wambutt R.;
RT   "Construction of a new Escherichia coli - Saccharomyces cerevisiae
RT   shuttle plasmid cloning vector allowing positive selection for
RT   cloned fragments";
RL   Folia Microbiol. 37:193-198(1992).
RN   [2]
RC   pWM8 from amp gene & yeast 2 micron & URA3 gene
RA   Wambutt R., Mashko S.V.;
RT   ;
RL   Unpublished (1992).
RN   [3]
RC   pML24 from amp gene
RA   Mashko S.V., Lebedeva M.I., Epishin S.M., Vinetskii I.P.,
RA   Ketlinskii S.A., Debabov V.G.;
RT   "[Synthesis of biologically active recombinant human
RT   interleukin-1alpha in Escherichia coli cells]";
RL   Dokl. Akad. Nauk. 324:706-710(1992).
RN   [4]
RC   pDB262 from pTR262 & pDB248, LEU2 gene/ARS
RC   pWH4 from pDB262 & pUN121
RC   pWH5 from pWH4 & pUN121
RC   pMAK262 from pTR262 & LEU2 gene
RC   pDAM3 from pBR322 & LEU2 gene
RC   pDAM6 from pBR325 & YEp13, LEU2 gene
RA   Wright A., Maundrell K., Heyer W.D., Beach D., Nurse P.;
RT   "Vectors for construction of gene banks and integration of cloned
RT   genes in Schizosaccharomyces pombe and Saccharomyces cerevisiae";
RL   Plasmid 15:156-158(1986).
RN   [5]
RC   TGATG from pBR322
RA   Mashko S.V., Veiko V.P., Lapidus A.L., Lebedeva M.I., Mochulsky A.V.,
RA   Shechter I.I., Trukhan M.E., Ratmanova K.I., Rebentish B.A.,
RA   Kaluzhsky V.E.;
RT   "TGATG vector: a new expression system for cloned foreign genes in
RT   Escherichia coli cells";
RL   Gene 88:121-126(1990).
CC   NM (pPW262)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUN121)(pHC624)(pBR327)(pWM8)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove, tet gene
FT                   -> pML24 3300bp
FT                   1. pBR322 4361bp, ori/amp gene
FT                   2. yeast 2 micron HindIII-EcoRI 1390bp 1018..2408, ori
FT                   3. yeast HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> pWM8 7000bp
FT                   1. pBR327 BamHI-EcoRI 2896bp 376..3272
FT                   2. pWM8 EcoRI-BamHI 3300bp, 2 micron ori/URA3 gene
FT                   -> pPW2 6300bp
FT                   1. pPW2 EcoRI 6300bp
FT                   Klenow
FT                   -> pPW26 6300bp
FT                   1. pML24 BamHI-HindIII, ori/amp gene
FT                   2. pHC624 remove short BamHI-HindIII, MCS
FT                   -> pMLP2 2500bp
FT                   1. pMLP2 remove PstI-BamHI, MCS/2500bp
FT                   2. pUN121 PstI-BamHI 1500bp, cI857/cro
FT                   -> pMLP21 4000bp
FT                   1. pPW26 BamHI 6300bp
FT                   2. pMLP21 BamHI-BglII 1500bp, cI857/cro
FT                   -> pPW262 7800bp"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="GEN yeast URA3 gene"
FT   CDS             0..0
FT                   /note="GEN lambda phage cI857 gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;