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ID   PRB58      preliminary; circular DNA; SYN; 6000 BP.
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AC   IG7030;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Saccharomyces plasmid vector pRB58 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRB51, pRB52, pRB53, pRB54, pRB55 from YEp24 & yeast SUC2 gene
RC   pRB56, pRB57 from YEp24 & yeast SUC2 gene
RC   from pBR322 & pRB52
RC   pRB58, pRB60 from pRB55
RA   Carlson M., Botstein D.;
RT   "Two differentially regulated mRNAs with different 5' ends encode
RT   secreted with intracellular forms of yeast invertase";
RL   Cell 28:145-154(1982).
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CC   NM (pRB58)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF (pSMF2)(pSMF3)(pSMF4)(pSMF6)
CC   OR (yes)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp24 BamHI
FT                   calf intestinal phosphatase
FT                   2. yeast genome S288C Sau3A1-Sau3A1, SUC2 gene
FT                   -> pRB55
FT                   1. pRB55 recombination/gene conversion
FT                   -> pRB58"
FT   misc_feature    0..0
FT                   /note="SUC2 5'"
FT   misc_feature    0..0
FT                   /note="XhoI"
FT   misc_feature    0..0
FT                   /note="AvaII"
FT   misc_feature    0..0
FT                   /note="EcoRI"
FT   misc_feature    0..0
FT                   /note="SUC2"
FT   misc_feature    0..0
FT                   /note="AvaII"
FT   misc_feature    0..0
FT                   /note="BamHI"
FT   misc_feature    0..0
FT                   /note="XbaI"
FT   misc_feature    0..0
FT                   /note="SUC2 3'"
FT   misc_feature    0..0
FT                   /note="Sau3A"
FT   misc_feature    0..0
FT                   /note="HindIII"
FT   misc_feature    0..0
FT                   /note="EcoRI"
FT   misc_feature    0..0
FT                   /note="URA3"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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