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ID   PRIT17     preliminary; circular DNA; SYN; 1 BP.
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AC   IG7033;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli phagemid vector pRIT17 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRIT17, pRIT18 from pRIT4 & IGF-I gene
RC   plasmid from pRIT15
RA   Moks T., Abrahmsen L., Holmgren E., Bilich M., Olsson A.,
RA   Uhlen M., Pohl G., Sterky C., Hultberg H., Josephson S.,
RA   Holmgren A., Joernvall H., Nilsson B.;
RT   "Expression of human insulin-like growth factor I in bacteria: use
RT   of optimized gene fusion vectors to facilitate protein purification";
RL   Biochemistry 26:5239-5244(1987).
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RN   [2]
RC   pRIT14 from pASEE & IGF-1 gene
RC   pRIT15 from pRIT14 & pEMBL8
RC   pRIT16 from pRIT4 & Staphylococcus protein A
RA   Abrahmsen L., Moks T., Nilsson B., Uhlen M.;
RT   "Secretion of heterologous gene products to the culture medium of
RT   Escherichia coli";
RL   Nucleic Acids Res. 14:7487-7500(1986).
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RN   [3]
RC   pRIT11, pRIT12, pRIT13 from pRIT4 & linker
RC   pRIT17 from pRIT12 & phoA gene
RA   Lowenadler B., Nilsson B., Abrahmsen L., Moks T., Ljungqvist L.,
RA   Holmgren E., Paleus S., Josephson S., Philipson L., Uhlen M.;
RT   "Production of specific antibodies against protein A fusion
RT   proteins";
RL   EMBO J. 5:2393-2398(1986).
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RN   [4]
RC   pASE3 or pASE from M13mp8 & Staphylococcus protein A
RA   Abrahmsen L., Moks T., Nilsson B., Hellman U., Uhlen M.;
RT   "Analysis of signals for secretion in the staphylococcal protein A
RT   gene";
RL   EMBO J. 4:3901-3905(1985).
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RN   [5]
RC   pASEE from M13mp8 & Staphylococcus protein A
RA   Moks T., Abrahmsen L., Nilsson B., Hellman U., Sjoquist I., Uhlen M.;
RT   "Staphylococcal protein A consists of five IgG-binding domains";
RL   Eur. J. Biochem. 156:637-643(1986).
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RN   [6]
RC   pUN201 from Staphylococcus protein A gene
RA   Nilsson B., Holmgren E., Josephson S., Gatenbeck S., Philipson L.,
RA   Uhlen M.;
RT   "Efficient secretion and purification of human insulin-like growth
RT   factor I with a gene fusion vector in Staphylococci";
RL   Nucleic Acids Res. 13:1151-1162(1985).
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CC   NM (pRIT17)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE (affinity)
CC   PA (pRIT4)(pRIT15)
CC   BR (pRIT18)
CC   OF ()
CC   OR (no)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRIT4 remove small EcoRI-HindIII
FT                   2. pUC8::modified IGF-I gene EcoRI-HindIII, IGF-I gene
FT                   -> pRIT17"
FT   misc_feature    0..0
FT                   /note="signal peptide"
FT   misc_feature    0..0
FT                   /note="IgG-binding domain E"
FT   misc_feature    0..0
FT                   /note="SIT hydroxylamine cleavage site"
FT   misc_feature    0..0
FT                   /note="IgG-binding domain D"
FT   misc_feature    0..0
FT                   /note="SIT hydroxylamine cleavage site"
FT   misc_feature    0..0
FT                   /note="IgG-binding domain A"
FT   misc_feature    0..0
FT                   /note="SIT hydroxylamine cleavage site"
FT   misc_feature    0..0
FT                   /note="IgG-binding domain B"
FT   misc_feature    0..0
FT                   /note="SIT hydroxylamine cleavage site"
FT   misc_feature    0..0
FT                   /note="IgG-binding domain C"
FT   misc_feature    0..0
FT                   /note="SIT hydroxylamine cleavage site"
FT   misc_feature    0..0
FT                   /note="in pRIT15 only, there are 2 IgG-binding
FT                   domain E, replacing E through C"
FT   misc_feature    0..0
FT                   /note="in pRIT18 only, there is 6 residues of E plus
FT                   2 IgG-binding domain Z, replacing E through C"
FT   misc_feature    0..0
FT                   /note="SIT hydroxylamine cleavage site"
FT   misc_feature    0..0
FT                   /note="human IGF-I gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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