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ID   PRK2013    preliminary; circular DNA; SYN; 4800 BP.
AC   IG1110; ATCC37159; X04996; X04995; V00087; J01541; M61152; M38354;
AC   S54410; M14641;
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pRK2013 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pRK212.1 from RK2
RC   pRK212.2 from RK2
RC   pRK214.1 from RK2
RC   pRK215.1 from RK2
RC   pDF37 from pML2 & trpE gene
RC   pRK2013, pRK2023 from pDF1 & pRK212.1, RK2 oriT
RC   pRK2067 from pRK2023 & pRK212.1
RC   pRK2076 from pRK2023 & pRK2067 & pDF37
RC   pRK2080 from pRK2076
RC   pRK256 from RK2
RC   pRK2045 from pSC101 & pRK212.1
RC   [pRK2073 from pRK2013 & Tn7]
RA   Figurski D.H., Helinski D.R.;
RT   "Replication of an origin-containing derivative of plasmid RK2
RT   dependent on a plasmid function provided in trans";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:1648-1652(1979).
RN   [2]
RC   pRi8196 from Agrobacterium rhizogenes
RC   from pBR328 & pRi8196
RA   Koplow J., Byrne M.C., Jen G., Tempe J., Chilton M.D.;
RT   "Physical map of the Agrobacterium rhizogenes strain 8196 virulence
RT   plasmid";
RL   Plasmid 11:17-27(1984).
CC   This is a helper plasmid for mobilization of non-self-transmissible
CC   plasmids. (ATCC staff)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pRK2013)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(E.coli)(Agrobacterium)
CC   CP ()
CC   FN (tri-parental mating)(conjugation)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. RK2
FT                   integration
FT                   2. phage Mu
FT                   -> pRK212
FT                   1. RK2 remove HindIII-HindIII 6700bp 30400..37100, kan
FT                   2. phage Mu 1000bp
FT                   -> pRK212.1 49700bp
FT                   [oriT/EcoRI in RK2/unique HindIII]]
FT                   1. mini-F EcoRI
FT                   2. E. coli EcoRI-EcoRI, kan gene
FT                   -> pML31
FT                   1. pRK212.1 SalI
FT                   2. pML31 SalI-SalI, kan gene
FT                   -> pRK212.2 56100bp
FT                   1. ColE1 EcoRI 6646bp
FT                   2. mini-F EcoRI-EcoRI
FT                   -> pDF1
FT                   1. pDF1 EcoRI-EcoRI 6300bp, ori/no amp
FT                   2. pRK212.2 EcoRI-EcoRI 41700bp, RK2 oriT/kan
FT                   -> pRK2013 4800bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;