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ID   PRS202     preliminary; circular DNA; SYN; 6270 BP.
AC   ATCC77167;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli phagemid vector pRS202 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pRSS56 from pBluescript KS+ & BlueScribe
RC   pRS303 from pRSS56 & HIS3 gene
RC   pRS304 from pRSS56 & TRP1 gene
RC   pRS305 from pRSS56 & LEU2 gene
RC   pRS306 from pRSS56 & URA3 gene
RC   pUC19-CEN6:32 from pUC19 & CEN6
RC   pRSS83 from pUC19-CEN6:32 & pAB9, ARS
RC   pRSS84 from pRSS83
RC   pRS313 from pRS303 & pRSS84
RC   pRS314 from pRS304 & pRSS84
RC   pRS315 from pRS305 & pRSS84
RC   pRS316 from pRS306 & pRSS84
RC   pRSS93 from pRSS84 & pYCF5
RA   Sikorski R.S., Hieter P.;
RT   "A system of shuttle vectors and yeast host strains designed for
RT   efficient manipulation of DNA in Saccharomyces cerevisiae";
RL   Genetics 122:19-27(1989).
CC   The order of the major features in this plasmid is: URA3 - f1 ori
CC   (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla -
CC   2 micron ori. (personal communication)
CC   YE-type shuttle vector permitting visual detection of recombinants and
CC   production of ssDNA in E.coli. Contains promoters for in vitro RNA
CC   synthesis, priming sites useful for sequencing, and encodes the lacZ
CC   alpha (lacZ') peptide. (personal communication)
CC   pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of
CC   pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains
CC   the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites
CC   between bla and f1 origin of pBS(+).
CC   Constructed from pRS306 (ATCC 77141) by inserting a 1.891 kb fragment
CC   from the 2 micron plasmid into the AatII site and inserting BglII
CC   linkers into the SmaI site of the polylinker. (personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   PvuI--3.7, 1.9, 0.7; HindIII--6.3; EcoRV--4.8, 1.5; XbaI--3.5, 2.7;
CC   PvuII--5.9, 0.4. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pRS202)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pRS306 AatII 4373bp 4309..4309
FT                   2. yeast 2 micron 1891bp, ori
FT                   -> plasmid 6264bp
FT                   1. plasmid SmaI 6264bp 2038..2038, MCS
FT                   BglII linker 6bp agatct
FT                   -> pRS202 6270bp"
FT   misc_binding    0..0
FT                   /note="MCS KpnI-ApaI-XhoI-SalI-ClaI-HindIII-EcoRV-
FT                   EcoRI-PstI-BglII-
FT                   BamHI-SpeI-XbaI-EagI-NotI-BstXI-SacII-SacI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-ApaI-XhoI-SalI-ClaI-EcoRI-
FT                   PstI-BglII-BamHI-SpeI-EagI-NotI-SacII-SacI-NaeI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS H4"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron ori"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;