ID   PRW1724    preliminary; circular DNA; SYN; 66 BP.
XX
AC   M35458;
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DT   15-SEP-1990 (Rel. 5, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pRW1724 - incomplete, polypurine-pyrimidine.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-66
RC   pRW1707 from pRW1703 & pRW1704 & pRW791
RC   pRW1724 from pRW1411 & pRW1408 & pRW791
RC   pRW1725 from pRW1411 & pRW1408 & pRW791
RC   pRW1718 from pRW1707
RA   Shimizu M., Hanvey J.C., Wells R.D.;
RT   "Multiple non-B-DNA conformations of polypurine-polypyrimidine
RT   sequences in plasmids";
RL   Biochemistry 29:4704-4713(1990).
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CC   NCBI gi: 207751
CC   NM (pRW1724)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pRW1708)(pRW1711)(pRW791 from pBR322)
CC   BR (pRW1707)(pRW1725)(pRW777)
CC   OF ()
CC   OR (no)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pRW790 1991bp
FT                   1. pRW790
FT                   2. linker
FT                   -> pRW1411
FT                   1. pRW1411 BamHI-BamHI, (GGA)8
FT                   -> fragment
FT                   1. fragment BamHI-BamHI
FT                   2. pRW791 BamHI
FT                   -> pRW1724"
FT   misc_feature    0..0
FT                   /note="SIT BamHI"
FT   misc_feature    0..0
FT                   /note="pRW1711 BamHI-BamHI
FT                   ggaggaggaggaggaggaggagga insert"
FT   misc_feature    0..0
FT                   /note="SIT BamHI"
FT   misc_feature    0..0
FT                   /note="pRW1711 BamHI-BamHI
FT                   ggaggaggaggaggaggaggagga insert"
FT   misc_feature    0..0
FT                   /note="SIT BamHI"
FT   misc_feature    0..0
FT                   /note="pRW791"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT                   from pRW791"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene,
FT                   from pRW791"
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SQ   Sequence 66 BP; 19 A; 6 C; 38 G; 3 T; 0 other;
     ggatccggag gaggaggagg aggaggagga ggatccggag gaggaggagg aggaggagga
     ggatcc
//