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ID   PRY107     preliminary; circular DNA; SYN; 6750 BP.
AC   IG8009;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pRY107 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pRY103 from pILL550
RC   pRY104 from pRY103
RC   pRY105, pRY106 from pRY103
RC   pRY107 from pRY106 & pWSK29
RC   pRY108 from pRY106 & pWSK30
RC   pRY109 from pUC18 & cat gene
RC   pRY110 from pRY105 & pRY109
RC   pRY111 from pRY110 & pWSK29
RC   pRY112 from pRY110 & pWSK30
RA   Yao R., Alm R.A., Trust T.J., Guerry P.;
RT   "Construction of new Campylobacter cloning vectors and a new
RT   mutational cat cassette";
RL   Gene 130:127-130(1993).
RN   [2]
RC   pWSK29 from pBluescript II SK+ & pLG339
RC   pWSK30 from pBluescript II SK- & pLG339
RC   pWSK129 from pWSK29 & pLG339
RC   pWSK130 from pWSK30 & pLG339
RA   Wang Y., Kushner S.R.;
RT   "Construction of versatile low-copy-number vectors for cloning,
RT   sequencing, and gene expression in Escherichia coli";
RL   Gene 100:195-199(1991).
CC   NM (pRY107)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Campylobacter coli, ori
FT                   -> pIP1455
FT                   1. pBR322 3100bp, ori
FT                   2. pIP1455 3240bp, C.coli
FT                   3. C.coli 1400bp, kan gene
FT                   4. C.coli, 760bp, oriT
FT                   -> pILL550 8500bp
FT                   1. pBluescript II SK+ 2961bp
FT                   PCR
FT                   BglII-BglII 2300bp, amp gene/lacZ gene
FT                   2. pLG339 PstI-SalI, pSC101 ori
FT                   -> pWSK29
FT                   1. pILL550 EcoRI-SalI 5500bp, kan gene/oriT/pIP1455
FT                   Klenow:Klenow
FT                   2. pBR328 AvaI-PstI 1099bp 1426..2525
FT                   Klenow:Klenow
FT                   -> pRY103 6600bp
FT                   1. pRY103 AccI 6600bp, pBR328 653..653
FT                   fill in
FT                   ClaI, pBR328 25..25
FT                   fill in
FT                   -> pRY106 6600bp
FT                   1. pRY106 remove small HincII-SmaI 1000bp, 5600bp
FT                   2. pWSK29 HincII-SmaI 1150bp, MCS/f1 ori
FT                   \ pBluescript II SK+ 677..-..716
FT                   -> pRY107 6750bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;