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ID   PSB34      preliminary; circular DNA; SYN; 1 BP.
XX
AC   ATCC56076;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Zygosaccharomyces plasmid vector pSB3 and pSB4 - incomplete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSB1 from S. bailii IFO0488
RC   pSB2 from S. bailii IFO1047
RC   pSB3, pSB4 from S. bisporus IFO1730
RC   pAT71 from YIp5 & pSB1
RC   pAT73 from YIp5 & pSB1
RC   pAT63 from YIp5 & pSB2
RC   pAT65 from YIp5 & pSB2
RC   pAT89 from YIp5 & pSB3
RC   pAT115 from YIp5 & pSB3
RC   pAT129 from YIp5 & pSB3
RC   pAT165 from YIp5 & pSB3
RC   pAT88 from YIp5 & pSB4
RA   Toh-e A., Araki H., Utatsu I., Oshima Y.;
RT   "Plasmids resembling 2-micrometers DNA in the osmotolerant yeasts
RT   Saccharomyces bailii and Saccharomyces bisporus";
RL   J. Gen. Microbiol. 130:2527-2534(1984).
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CC   Natural isolates from S. bisporus IFO 1730 possessing an ARS sequence
CC   effective in S. cerevisiae.
CC   Medium is 200 YM agar or broth.
CC   NM (pSB3 AND pSB4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Zygosaccharomyces rouxii IFO 1730)(Zygosaccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. S. bisporus IFO1730
FT                   -> pSB3"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-XhoI"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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