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ID   PSE640     preliminary; circular DNA; SYN; 9000 BP.
AC   ATCC77272;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pSE640 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pNKY3 from YEp24 & linker
RC   pNKY49 from pNK294 & pNKY3
RC   pNKY50 from pNKY49 & pNK294
RC   pNKY51 from pNKY50
RC   pNKY1006 from YRp7 & linker
RC   pNKY1009 from pNKY1006 & pNKY51
RA   Alani E., Cao L., Kleckner N.;
RT   "A method for gene disruption that allows repeated use of URA3
RT   selection in the construction of multiply disrupted yeast
RT   strains";
RL   Genetics 116:541-545(1987).
CC   YC-type shuttle enhancer-cloning vector using beta-galactosidase
CC   activity as the reporter. (personal communication)
CC   The order of the major features in this plasmid is: MCS - CYC - lacZ
CC   - pMB1 ori - ampR - URA3 - ARS1 - SUP11 - CEN4. (personal
CC   communication)
CC   The UAS has been deleted from the CYC1 promoter, so expression of a
CC   CYC1-lacZ fusion protein depends on UAS elements cloned into
CC   polylinker region. (personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--9.2; XhoI--9.2; SalI--9.2. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSE640)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp
FT                   2. yeast 6300bp, ARS1/CYC/URA3/CEN4/SUP11
FT                   -> pSE640 9000bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-EcoRI-SacI-KpnI-
FT                   SmaI-XhoI"
FT   misc_binding    0..0
FT                   /note="SIT unique SphI-SalI-EcoRI-XhoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN4"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="ANT yeast SUP11 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;