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ID   PSKKAN1105 preliminary; circular DNA; SYN; 75 BP.
AC   M22410;
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pSK-kan1105 - incomplete, fusion.
KW   cloning vector; aminiphosphotransferase 3'-phosphotransferase I;
KW   phosphotransferase.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-75
RC   pUC-URA3 from pUC19 & pG63
RC   pUK-S11 from pUC-URA3 & A15
RC   pUK-kan230 from pUK-S11 & Tn903, kan gene
RC   pUK-kan133 from pUK-S11 & pUK-kan230, APH1 gene
RC   pSK-kan401 from pUK-kan133 & pLGSD5, yeast 2 micron ori
RC   pSK-kan1105 from pSK-kan401 & linker
RC   pSK-kan1238 from pSK-kan1105
RC   pKF111, pKF122, pKF215, pKF229 from pSK-kan401 & E1
RA   Chen X.J., Fukuhara H.;
RT   "A gene fusion system using the aminoglycoside
RT   3'-phosphotrans-ferase gene of the kanamycin-resistance transposon
RT   Tn903: Use in the yeasts Kluyveromyces lactis and Saccharomyces
RT   cerevisiae";
RL   Gene 69:181-192(1988).
RN   [2]
RP   1-75
RC   pUK-kan1105
RA   Fukuhara H.;
RT   ;
RL   Submitted (03-FEB-1989) by:
RL   Fukuhara H., .
CC   The first 19 codons of the APHI gene can
CC   be replaced by other genes in a continuous reading frame without
CC   loss of APH acitivity.  pSk-kan1105 is formed from pSK-kan401
CC   KANFUSA after digestion with BamHI and ligation to two synthetic
CC   complementary 11-mers (gatcctcgagc) with BamHI cohesive ends.
CC   Insertion, at the polylinker site, of a DNA fragment having
CC   promoter activity will restore APH activity.
CC   NCBI gi: 208697
CC   NM (pSK-kan1105)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSK-kan401)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pSK-kan401 BamHI 7900bp, MCS
FT                   \ YEp24 3785..3785
FT                   2. BamHI-XhoI linker 11bp gatcctcgagc
FT                   -> pSK-kan1105 7900bp"
FT   misc_feature    1..44
FT                   /note="MCS SacI-KpnI-BamHI-XhoI"
FT   CDS             70..>75
FT                   /note="GEN E. coli aminiglycoside
FT                   3'-phosphotransferase I gene;
FT                   kanamycin resistance gene (kan);
FT                   NCBI gi: 208698"
SQ   Sequence 75 BP; 18 A; 20 C; 21 G; 16 T; 0 other;
     gaattcgagc tcggtacccg gggatcctcg agcgatcctc tagagtcgag gccgcgatta
     aattccaaca tggat