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ID   PSKKAN401  preliminary; circular DNA; SYN; 64 BP.
AC   M22409;
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pSK-kan401 - incomplete, fusion.
KW   cloning vector; aminiglycoside 3'-phosphotransferase I;
KW   phosphotransferase I.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-64
RC   pUC-URA3 from pUC19 & pG63
RC   pUK-S11 from pUC-URA3 & A15
RC   pUK-kan230 from pUK-S11 & Tn903, kan gene
RC   pUK-kan133 from pUK-S11 & pUK-kan230, APH1 gene
RC   pSK-kan401 from pUK-kan133 & pLGSD5, yeast 2 micron ori
RC   pSK-kan1105 from pSK-kan401 & linker
RC   pSK-kan1238 from pSK-kan1105
RC   pKF111, pKF122, pKF215, pKF229 from pSK-kan401 & E1
RA   Chen X.J., Fukuhara H.;
RT   "A gene fusion system using the aminoglycoside
RT   3'-phosphotrans-ferase gene of the kanamycin-resistance transposon
RT   Tn903: Use in the yeasts Kluyveromyces lactis and Saccharomyces
RT   cerevisiae";
RL   Gene 69:181-192(1988).
RN   [2]
RP   1-64
RC   pSK-kan401
RA   Fukuhara H.;
RT   ;
RL   Submitted (03-FEB-1989) by:
RL   Fukuhara H., .
RN   [3]
RC   pG6 from yeast 2 micron ori & pBR322
RC   pG63 from pG6 & URA3 gene
RC   pG63.11 from pG63
RC   pHCG3 from pG63.11 & pHC79
RA   Gerbaud C., Elmerich C., Tandeau De Marsac N.T., Chocat P.,
RA   Charpin N., Guerineau M., Aubert J.P.;
RT   "Construction of new yeast vectors and cloning of the Nif (nitrogen
RT   fixation) gene cluster of Klebsiella pneumoniae in yeast";
RL   Curr. Genet. 3:173-180(1981).
RN   [4]
RC   A10, A12, A15 from YIp5 & pKD1, ori
RC   E1, P1, B11 from YIp5 & pKD1, B form
RC   P3 from YIp5 & pKD1, A form
RC   S11 from YIp5 & pKD1
RC   HP1 from YIp5 & pKD1
RC   pCXJ1 from pUC19 & pKD1 & URA3 gene
RC   pCXJ-kan1 from pCXJ1 & Tn903 kan gene
RA   Bianchi M.M., Falcone C., Chen X.J., Wesolowski-Louvel M.,
RA   Fontali L., Fukuhara H.;
RT   "Transformation of the yeast Kluveromyces lactis by new vectors
RT   derived from the 1.6 micron circular plasmid pKD1";
RL   Curr. Genet. 12:185-192(1987).
RN   [5]
RC   pCE1 from E. coli IncP plasmids
RA   Elmerich C., Houmard J., Sibold L., Manheimer I., Charpin N.;
RT   "Genetic and biochemical analysis of mutants induced by
RT   bacteriophage Mu DNA integration into Klebsiella pneumoniae
RT   nitrogen fixation genes";
RL   Mol. Gen. Genet. 165:181-189(1978).
CC   The first 19 codons of the APHI gene can
CC   be replaced by other genes in a continuous reading frame without
CC   loss of APH acitivity.  The ATG start codon at position 59 is the
CC   second ATG start downstream from the potential RBS of the APHI
CC   gene.  Insertion, at the polylinker site, of a DNA fragment having
CC   a promoter activity will restore APH activity.
CC   NCBI gi: 208695
CC   NM (pSK-kan401)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII-EcoRI 4330bp 30..4360
FT                   2. yeast 2 micron B form EcoRI-HindIII 2131bp
FT                   \ 2408..3942 & 703..106, ori
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   -> pG6 6461bp
FT                   1. pG6 HindIII 6461bp 30..30
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> pG63 7550bp
FT                   1. pUC19 NarI 2686bp 237..237, 3' lacZ gene
FT                   Klenow
FT                   2. pG63 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   Klenow:Klenow
FT                   -> pUC-URA3 3800bp
FT                   1. YIp5 5541bp
FT                   2. pKD1, ori/4757bp-
FT                   -> A15 6000bp
FT                   1. pUC-URA3 AatII 3800bp, pUC19 2622..2622
FT                   Klenow
FT                   2. A15 Sau3AI-Sau3AI 914bp, s11 fragment/pKD1 ori
FT                   Klenow:Klenow
FT                   alkaline phosphatase
FT                   -> pUK-S11 4700bp
FT                   1. pUK-S11 SalI 4700bp, MCS/pUC19 418
FT                   2. pACYC177 HaeII-HaeII 1430bp 1596..3026, Tn903 kan
FT                   -> pUK-kan230 6100bp
FT                   1. pUK-S11 SalI 4700bp, MCS/pUC19 418
FT                   2. pUK-kan230 XhoI-SalI 1400bp, kan gene
FT                   -> pUK-kan133 6100bp
FT                   1. YEp24 7769bp, ori
FT                   2. yeast, CYC1 promoter
FT                   3. E. coli, lacZ gene
FT                   -> pLG669-Z
FT                   1. pLG669-Z
FT                   2. yeast, GAL1-GAL10 promoter
FT                   -> pLGSD5
FT                   1. pUK-kan133 EcoRI 6100bp, pUC19 397
FT                   2. pLGSD5 EcoRI-EcoRI 2000bp, yeast 2 micron ori
FT                   -> pSK-kan401 8100bp"
FT   misc_feature    1..33
FT                   /note="MCS SacI-KpnI-BamHI"
FT   CDS             59..>64
FT                   /note="GEN E. coli aminiglycoside
FT                   3'-phosphotransferase I gene;
FT                   kanamycin resistance gene (kan);
FT                   NCBI gi: 208696"
SQ   Sequence 64 BP; 16 A; 16 C; 18 G; 14 T; 0 other;
     gaattcgagc tcggtacccg gggatcctct agagtcgagg ccgcgattaa attccaacat