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ID   PSMF38T    preliminary; circular DNA; SYN; 6600 BP.
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AC   IG7036;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pSMF38T - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSMF2, pSMF3, pSMF4 from pUC8 & pRB58 & BamHI linker
RC   pSMF6 from pUC8 & pRB58 & BamHI linker
RC   pSMF12 from pSMF2 & pSMF6 & pBR322
RC   pSMF15 from pSMF3 & pSMF6 & pBR322
RC   pSMF18 from pSMF4 & pSMF6 & pBR322
RC   pSMF32T from pSMF12 & yeast 2 micron
RC   pSMF35T from pSMF15 & yeast 2 micron
RC   pSMF38T from pSMF18 & yeast 2 micron
RC   pSMF38TMA from pSMF38T & pMSa104, mouse amylase gene
RC   pSMF69MA from pSMF38MA
RA   Nishizawa M., Ozawa F., Hishinuma F.;
RT   "Construction of an expression and secretion vector for the yeast
RT   Saccharomyces cerevisiae";
RL   Agric. Biol. Chem. 51:515-521(1987).
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CC   NM (pSMF38T)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Saccharomyces cerevisiae)(E. coli)
CC   CP ()
CC   FN (cloning)(secretion)
CC   SE ()
CC   PA (pSMF18 from pSMF4)(2 micron plasmid)(YRp7 with TRP1)
CC   BR (pSMF32T from pSMF12)(pSMF35T from pSMF15)
CC   OF (pSMF38MA)(pSMF69MA)
CC   OR (yes)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRB58 AvaII-AvaII 1400bp, SUC2 gene
FT                   Klenow, fill in:Klenow, fill in
FT                   BamHI linker 12 mer:BamHI linker 12 mer
FT                   EcoRI-BamHI 1000bp
FT                   2. pUC8 BamHI-EcoRI 3000bp
FT                   -> pSMF4
FT                   1. pRB58 Sau3A-HindIII 600bp, SUC2 gene
FT                   Klenow, fill in:Klenow, fill in
FT                   BamHI linker 10mer:BamHI linker 10mer
FT                   BamHI-AluI 577bp
FT                   2. pUC8 HincII-BamHI 3000bp
FT                   -> pSMF6
FT                   1. pSMF6 BamHI-HindIII 580bp
FT                   2. pBR322 PvuII
FT                   HindIII linker
FT                   HindIII-EcoRI 2300bp
FT                   3. pSMF4 EcoRI-BamHI 1000bp
FT                   -> pSMF18 3800bp
FT                   1. pBR322 EcoRI
FT                   2. pDB248 EcoRI-PstI 1000bp
FT                   3. YRp7 PstI-EcoRI 1000bp
FT                   -> plasmid
FT                   1. plasmid EcoRI-EcoRI 2800bp
FT                   2. pSMF18 EcoRI
FT                   -> pSMF38T 6600bp"
FT   misc_feature    0..0
FT                   /note="SIT EcoRI"
FT   misc_feature    0..0
FT                   /note="SUC2 5' EcoRI-BamHI 1000bp
FT                   has SUC2 promoter, signal sequence, and 11 AA"
FT   misc_feature    0..0
FT                   /note="SIT BamHI"
FT   misc_feature    0..0
FT                   /note="SUC2 3' BamHI-HindIII 800bp
FT                   has 3 stop codons and terminator"
FT   misc_feature    0..0
FT                   /note="SIT HindIII"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli amp gene"
FT   misc_feature    0..0
FT                   /note="SIT EcoRI"
FT   misc_feature    0..0
FT                   /note="SIT EcoRI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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