back Return to this vector's summary.
ID   PSMF69MA   preliminary; circular DNA; SYN; 351 BP.
XX
AC   D10338; D00123; D10337; D00122;
XX
DT   23-JUL-1992 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   Saccharomyces plasmid vector pSMF69MA - incomplete, mouse amylase.
XX
KW   cloning vector; invertase; amylase; amylase fusion protein.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-148, D10338; D00123;
RP   1-111, D10337; D00122;
RC   pSMF2, pSMF3, pSMF4 from pUC8 & pRB58 & BamHI linker
RC   pSMF6 from pUC8 & pRB58 & BamHI linker
RC   pSMF12 from pSMF2 & pSMF6 & pBR322
RC   pSMF15 from pSMF3 & pSMF6 & pBR322
RC   pSMF18 from pSMF4 & pSMF6 & pBR322
RC   pSMF32T from pSMF12 & yeast 2 micron
RC   pSMF35T from pSMF15 & yeast 2 micron
RC   pSMF38T from pSMF18 & yeast 2 micron
RC   pSMF38TMA from pSMF38T & pMSa104, mouse amylase gene
RC   pSMF69MA from pSMF38MA
RA   Nishizawa M., Ozawa F., Hishinuma F.;
RT   "Construction of an expression and secretion vector for the yeast
RT   Saccharomyces cerevisiae";
RL   Agric. Biol. Chem. 51:515-521(1987).
XX
RN   [2]
RC   pMSa104 from mouse amylase gene
RA   Schibler U., Tosi M., Pittet A.C., Fabiani L., Wellauer P.K.;
RT   "Tissue-specific expression of mouse alpha-amylase genes";
RL   J. Mol. Biol. 142:93-116(1980).
XX
CC   Saccharomyces cerevisiae SUC2 gene (invertase) from pRB58.
CC   Construct pSMF69MA by removing a 37-bp Hind III fragment
CC   from pSMF38TMA, so that the fused protein product of
CC   pSMF69MA has no signal peptide, and remains inside the cells.
CC   The presence of the signal sequence of invertase
CC   may be necessary for the secretion of mouse amylase in yeast cells.
CC   NCBI gi: 220941 & 220943
CC   NM (pSMF69MA)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)(secretion)
CC   SE ()
CC   PA (pRB58)(pSMF38TMA)
CC   BR ()
CC   OF ()
CC   OR (no)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSMF38T remove HindIII-HindIII 37bp
FT                   -> pSMF69
FT                   1. pSMF69 BamHI
FT                   2. pMSa104 PstI-PstI, mouse amylase gene
FT                   PstI-ApaI, 5' end
FT                   S1 nuclease, blunt end
FT                   DraI-PstI, 3' end
FT                   BamHI linker 12mer
FT                   BamHI-BamHI
FT                   -> pSMF69MA
FT   mRNA            4..>148
FT                   /note="Saccharomyces cerevisiae SUC2 invertase gene
FT                   (from pRB58)/mouse amylase fusion protein; first"
FT   sig_peptide     44..100
FT                   /note="Saccharomyces cerevisiae SUC2 invertase gene
FT                   (from pRB58)/mouse amylase fusion protein; first"
FT   CDS             44..>148
FT                   /note="Saccharomyces cerevisiae SUC2 invertase gene
FT                   (from pRB58)/mouse amylase fusion protein;
FT                   NCBI gi: 220942; first"
FT   mat_peptide     101..>148
FT                   /note="Saccharomyces cerevisiae SUC2 invertase gene
FT                   (from pRB58)/mouse amylase fusion protein; first"
FT   mRNA            4..>111
FT                   /note="Saccharomyces cerevisiae SUC2 invertase gene
FT                   (from pRB58)/mouse amylase fusion protein; second"
FT   CDS             67..>111
FT                   /note="Saccharomyces cerevisiae SUC2 invertase gene
FT                   (from pRB58)/mouse amylase fusion protein;
FT                   NCBI gi: 220944; second"
XX
SQ   Sequence 351 BP; 80 A; 65 C; 41 G; 73 T; 92 other;
     aaacaagcaa aacaaaaagc ttttcttttc actaacgtat atgatgcttt tgcaagcttt
     ccttttcctt ttggctggtt ttgcagccaa aatatctgca tcaatgacaa acgaaactag
     cgatagacct ttggtccccg gatccccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     aaacaagcaa aacaaaaagc tttccttttc cttttggctg gttttgcagc caaaatatct
     gcatcaatga caaacgaaac tagcgataga cctttggtcc ccggatcccc a
//