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ID   PSP2       preliminary; DNA; SYN; 5580 BP.
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AC   ATCC77498;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Schizosaccharomyces/Saccharomyces/E.coli phagemid vector pSP2 - incom.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSP1 from pRS305
RC   pSP2 from pRS306
RA   Cottarel G.F., Beach D., Deuschle U.;
RT   "Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle
RT   vectors, pSP1 and pSP2";
RL   Curr. Genet. 23:547-548(1993).
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CC   Shuttle vector transforming S. pombe at high efficiency.  Suitable
CC   for generating nested deletions, DNA sequencing, and producing
CC   riboprobes. Permits visual detection of recombinants by lacZalpha
CC   complementation. Constructed from pRS306 by inserting a 1.2 kb EcoRI
CC   fragment containing ars1 into the AatII site.  The NdeI site in ars1
CC   was removed.
CC   The order of the major features (with directions of transcription) is:
CC   > URA3 - f1 - < lacZ' - SacI/MCS/KpnI - pMB1 ori - < ampR - ars1. [1]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: G.F.Cottarel
CC   NM (pSP2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Schizosaccharomyces pombe)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)(sequencing)(shuttle)(RNA synthesis in vitro)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRS306 AatII 4373bp 4309..4309,
FT                   \ amp/ori/LEU2/T3/T7/lac/f1
FT                   2. yeast NdeI, ARS1
FT                   fill in
FT                   EcoRI-EcoRI 1200bp, ARS1
FT                   -> pSP2 5580bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   misc_binding    0..0
FT                   /note="MCS KpnI-ApaI-XhoI-SalI-ClaI-HindIII-EcoRV-
FT                   EcoRI-PstI-SmaI-BamHI-SpeI-XbaI-EagI-NotI-SacII-
FT                   BstXI-SacI"
FT   misc_binding    0..0
FT                   /note="MCS unique KpnI-XhoI-SalI-ClaI-HindIII-EcoRI-
FT                   SmaI-BamHI-SpeI-EagI-NotI-SacII-BstXI-SacI"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
FT   promoter        0..0
FT                   /note="PRO phage T3"
FT   promoter        0..0
FT                   /note="PRO phage T7"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ' gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI phage f1"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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