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ID PSRW25 preliminary; circular DNA; SYN; 12600 BP.
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AC ATCC37506;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE E. coli plasmid vector pSRW25 - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC plasmid from pWT571 & pUR3
RC pWT701 from plasmid & pACYC184
RC pSRW10 from pRK2501 & pRPG16
RC pSRW17 from pWT701 & pRK2501
RC pSRW14, pSRW18 from pSRW10 & pWT701
RC pSRW21 from pRPG16 & pBR322
RC pSRW23 from pSRW21 & pSRW17
RC pSRW25 from pSRW21 & pRK2501
RC pI-24 from IFN-beta & pWT501
RC pMN45 from pI-24 & pMN38
RA Warne S.R., Thomas C.M., Nugent M.E., Tacon W.;
RT "Use of a modified Escherichia coli trpR gene to obtain tight
RT regulation of high-copy-number expression vectors";
RL Gene 46:103-112(1986).
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RN [2]
RC pLF1 from pAT153 & pUB110
RC pUR1 from pLF1 & urogastrone gene right half
RC pUR2 from pUR1 & linker
RC pUR3 from pUR2 & urogastrone gene left half
RA Smith J., Cook E., Fotheringham I., Pheby S., Derbyshire R.,
RA Eaton M.A.W., Doel M., Lilley D.M.J., Pardon J.F., Patel T.,
RA Lewis H., Bell L.D.;
RT "Chemical synthesis and cloning of a gene for human
RT beta-urogastrone";
RL Nucleic Acids Res. 10:4467-4481(1982).
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RN [3]
RC lambda thr trpR1 from lambda & trp gene & thr gene
RC pRPG2, pRPG3 from pACYC184 & lambda thr trpR1
RC pRPG1 from pACYC184 & lambda thr trpR1
RC pRPG4 from pMK16 & lambda thr trpR1
RC pRPG8 from pMK16 & lambda thr trpR1
RC pRPG12, pRPG14, pRPG16 from pHUB4 & pRPG4
RC pRPG6, pRPG7 from pRPG4
RA Gunsalus R.P., Zurawski G., Yanofsky C.;
RT "Structural and functional analysis of cloned deoxyribonucleic
RT acid containing the trpR-thr region of the Escherichia coli
RT chromosome";
RL J. Bacteriol. 140:106-113(1979).
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RN [4]
RC [pWT111cop from pWT series]
RC pMN38 from pWT111cop
RA Nugent M.E., Primrose S.B., Tacon W.C.;
RT "The stability of recombinant DNA";
RL Develop. Indust. Microbiol. 24:271-285(1983).
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RN [5]
RC pSRW101 from pSRW series
RA Pinkney M., Theophilus B.D., Warne S.R., Tacon W.C., Thomas C.M.;
RT "Analysis of the transcription from the trfA promoter of broad host
RT range plasmid RK2 in Escherichia coli, Pseudomonas putida and
RT Pseudomonas aeruginosa";
RL Plasmid 17:222-232(1987).
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CC Constructed to provide tight but inducible regulation from the trp
CC promoter on a high copy number plasmid.
CC Medium is 1273 LB plus tetracycline.
CC NM (pSRW25)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli K-12)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda
FT insertion
FT 2. E. coli, trp gene/thr gene
FT -> lambda thr trpR1
FT 1. pMK16 BamHI, tet gene
FT 2. lambda thr trpR1 BamHI-BamHI 1300bp, trp repressor
FT -> pRPG4 5800bp
FT 1. pHUB4 BamHI
FT 2. pRPG4 BamHI-BamHI 1300bp, trp repressor
FT -> pRPG16
FT 1. pRPG16 BamHI-BamHI 1300bp, trpR gene
FT remove small RsaI-RsaI
FT Klenow fill in
FT BamHI linker:
FT 2. pBR322 BamHI
FT -> pSRW21 5300bp
FT 1. pSRW21 EcoRI-SphI, trpR gene
FT Klenow fill in:Klenow fill in
FT 2. pRK2501 BglII
FT Klenow fill in
FT -> pSRW25 12600bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-XhoI-HindIII-ClaI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO E. coli trp gene"
FT CDS 0..0
FT /note="ANT E. coli kanamycin resistance gene (kan)"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
n
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