ID   PSV232ALAD preliminary; circular DNA; SYN; 1 BP.
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AC   M15077;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pSV232AL-Adelta5' - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [lambda gLuc1 from lambda & luc gene]
RC   [lambda Luc23 from lambda & luc gene]
RC   pLuc23-4 from pUC19 & lambda Luc23
RC   pLuc23A from pUC13 & lambda Luc23
RC   pLuc23B from pUC13 & lambda Luc23
RC   pJD180, pJD181, pJD182 from pUC19 & lambda gLuc1
RC   pJD183 from pUC13 & lambda gLuc1
RC   pJD200 from pUC18 & pJD180 & linker
RC   pJD201 from pJD200 & pLuc23-4
RC   pJD202 from pUC18 & pJD201
RC   pJD204, pJD206 from pJD202 & linker
RC   pJD205, pJD207 from pJD201 & linker
RC   pSV2A/L-Adelta5' from pSV2A & pJD206
RC   pSV232AL-Adelta5' from pSV232A & pJD206
RC   pRSVL-Adelta5' from pRSV & pJD206
RC   [pRSV-L from luciferase gene]
RA   De Wet J.R., Wood K.V., DeLuca M., Helinski D.R., Subramani S.;
RT   "Firefly luciferase gene: structure and expression in mammalian
RT   cells";
RL   Mol. Cell. Biol. 7:725-737(1987).
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CC   NM (pSV232AL-Adelta5')
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(vertebrate cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (SV40)(pBR322)
CC   BR ()
CC   OF (pXP1, pXP2, pXPBg, pXPB, pSluc2)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   2. luc gene
FT                   -> lambda gLuc1
FT                   1. lambda
FT                   2. luc gene
FT                   -> lambda Luc23
FT                   1. pUC19 EcoRI
FT                   2. lambda Luc23 EcoRI-EcoRI, luc gene
FT                   -> pLuc23-4
FT                   1. pUC19 EcoRI
FT                   2. lambda gLuc1 EcoRI-EcoRI 700bp, luc gene
FT                   -> pJD180
FT                   1. pUC18 HindIII
FT                   2. pJD180 EcoRI-EcoRI, luc gene
FT                   Klenow fill in:Klenow fill in
FT                   HindIII linker 10bp ccaagcttgg:HindIII linker 10bp
FT                   HindIII-HindIII
FT                   -> pJD200
FT                   1. pJD200 remove small XbaI-BamHI
FT                   2. pLuc23-4 large XbaI-BamHI
FT                   -> pJD201
FT                   1. pUC18 remove small HindIII-HincII
FT                   2. pJD201 HindIII-SspI, luc gene
FT                   -> pJD202
FT                   1. pJD202 BsmI-BsmI
FT                   T4 DNA polymerase fill in:T4 DNA polymerase fill in
FT                   HindIII linker:HindIII linker
FT                   HindIII-HindIII
FT                   -> pJD206 [L-Adelta5']
FT                   1. pSV232A HindIII-BglII
FT                   2. pJD206 HindIII-XbaI 1720bp, L-Adelta5' luc gene
FT                   -> pSV232A/L-Adelta5'"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_feature    0..0
FT                   /note="SV40"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="GEN firefly luciferase gene (luxA);
FT                   reporter gene"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   promoter        0..0
FT                   /note="ORI SV40; no enhancer"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//