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ID   PSV2APAP   preliminary; circular DNA; SYN; 1 BP.
AC   IG7042;
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Vertebrate/E.coli phagemid vector pSV2Apap - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   plasmid1 from pSV2Adhfr & PAP gene
RC   pSV2Apap from plasmid1 & M13mp19
RC   plasmid2 from pSV0Adhfr & PAP gene
RC   pSV0Apap from plasmid2 & M13mp19
RC   plasmid3 from pSV232Adhfr & PAP gene
RC   pSV232Apap from plasmid3 & M13mp19
RC   pSV2Acat from pSV2-cat & SV40 polyA
RA   Henthorn P., Zervos P., Raducha M., Harris H., Kadesch T.;
RT   "Expression of a human placental alkaline phosphatase gene in
RT   transfected cells: use as a reporter for studies of gene
RT   expression";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:6342-6346(1988).
RN   [2]
RC   plasmid from pSV2-gpt & linker
RC   pSV2Agpt from plasmid & SV40 polyA
RC   pSV232Agpt from pSV2Agpt & SV40 S-232 early gene promoter
RC   pSV2Aneo from pSV2-neo & linker & SV40 polyA
RC   pSV322neo from pBR322 & pSV2-neo
RC   pSV232Aneo from pSV322neo & linker & SV40 polyA
RC   pSV2Aneo2Agpt from pSV2Agpt & pSV2Aneo
RC   pSV232Aneo232Agpt from pSV232Agpt & pSV232Aneo
RC   pSV232Adhfr from pSV2-dhfr & linker & SV40 polyA
RC   pSV232A(neo/gpt/dhfr) from pSV232Aneo232Agpt & pSV232Adhfr
RC   pSV2Adhfr from pSV2-dhfr & linker & SV40 polyA
RC   pSV0Adhfr from pSV0-dhfr & linker & SV40 polyA
RA   Kadesch T., Berg P.;
RT   "Effects of the position of the simian virus 40 enhancer on
RT   expression of multiple transcription units in a single plasmid";
RL   Mol. Cell. Biol. 6:2593-2601(1986).
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSV1)
CC   BR (pSV0Apap)(pSV232Apap)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pSV2-dhfr PvuII
FT                   BamHI linker 10bp
FT                   BamHI
FT                   2. SV40 MboI-MboI 237bp, polyA MboI fragment G
FT                   BamHI-BglII
FT                   -> pSV2Adhfr [5171..270, promoter/ori/repeats]
FT                   1. pSV2Adhfr remove HindIII-BglII, dhfr gene
FT                   :fill in
FT                   2. human EcoRI-ScaI 4130bp, PAP gene
FT                   fill in:
FT                   HindIII linker
FT                   -> plasmid
FT                   1. plasmid remove BamHI-PstI, SV40
FT                   2. M13mp19 BamHI-PstI, MCS
FT                   -> pSV2Apap [unique XbaI, unique SalI]"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early gene"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;