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ID   PSV2HPH    preliminary; circular DNA; SYN; 5500 BP.
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AC   V01499; K01193; ATCC37647;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pSV2-hph - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pLG61, pLG62, pLG71, pLG72, pLG75, pLG76 from pJR225 & pBR322
RC   pLG83, pLG86, pLG89 from pEX-2 & pLG62
RC   pLG90 from hph gene
RC   pHygro from E. coli
RA   Gritz L., Davies J.;
RT   "Plasmid-encoded hygromycin B resistance: the sequence of hygromycin
RT   B phosphotransferase gene and its expression in Escherichia coli and
RT   Saccharomyces cerevisiae";
RL   Gene 25:179-188(1983).
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RN   [2]
RC   p236 from pBR322 & lambda, pL promoter
RA   Fiers W.;
RT   ;
RL   Unpublished (1983).
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RN   [3]
RC   pSV2hph from SV40 promoter/splice/polyA & linker & hph gene
RA   Berg P., Dieckmann M.;
RT   ;
RL   Unpublished (1993).
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CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII/BglII--3.87, 1.34, 0.69; PvuII/PstI--3.66, 1.84; PvuII--uncut.
CC   (ATCC staff)
CC   Consists of the SV40 promoter (SV nt 270-5171), a HindIII linker, the
CC   hph gene (1347 bp), SV40 splice site (SV nt 4710-4100), SV40
CC   polyadenylation site (SV nt 4362-2067), (personal communication from
CC   Paul Berg, Marianne Dieckmann)
CC   pML (inserted at the BamHI site with HindIII-EcoRI-amp upstream of the
CC   SV40 promoter). (personal communication from Paul Berg,
CC   Marianne Dieckmann)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSV2-hph)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 PvuII-EcoRI 2293bp 2067..4360,
FT                   \ ori/amp gene
FT                   2. SV40 HindIII-PvuII 344bp 5172..5243..273,
FT                   \ pro/spl/polyA
FT                   3. SV40 Sau3AI/MboI-Sau3AI/MboI 610bp 4100..4710,
FT                   \ pro/spl/polyA/complement
FT                   4. yeast 1347bp, hph/hyg gene
FT                   5. HindIII linker 10bp ccaagcttgg
FT                   -> pSV2hph 5500bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-BglI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT Saccharomyces hygromycin resistance gene
FT                   (hyg)"
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SQ   Sequence 1547 BP; 315 A; 421 C; 487 G; 324 T; 0 other;
     gagctcatga gcggagaacg agatgacgtt ggaggggcaa ggtcgcgctg attgctgggg
     caacacgtgg agcggatcgg ggattgtctt tcttcagctc gctgatgata tgctgacgct
     caatgccgtt tggcctccga ctaacgaaaa tcccgcattt ggacggctga tccgattggc
     acggcggacg gcgaatggcg gagcagacgc tcgtccgggg gcaatgagat atgaaaaagc
     ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta
     gatgccgacc gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct
     agcaaggcaa atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc
     gctaagctcg ctcggctggg tcgcgggagg gccggtcgca gtgattcagg cccttctgga
     ttgtgttggt ccccagggca cgattgtcat gcccacgcac tcgggtgatc tgactgatcc
     cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatct
//