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ID   PSVCA      preliminary; circular DNA; SYN; 59 BP.
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AC   K01425;
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DT   07-NOV-1984 (Rel. 1, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pSVCA - incomplete, RNA start site I.
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KW   cloning vector; conalbumin; recombinant DNA.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-59
RC   pSVCA, pSVCG from pHS102
RC   pTBCTB from pTCTB & pEMPB series
RC   pTCTCTB from pTCT & pTCTB
RC   pTB(CTB)3 from pTCTB & pEMPB series
RA   Wasylyk B., Wasylyk C., Augereau P., Chambon P.;
RT   "The SV40 72 bp repeat preferentially potentiates transcription
RT   starting from proximal natural or substitute promoter elements";
RL   Cell 32:503-514(1983).
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RN   [2]
RC   pDB14 from pBR322 & pHS102
RC   pTBO from pSV1
RC   pTB101, pTB202, pTB208 from pSV1
RC   pTBB1101 from pHS102 & pBR322
RC   pTBAS101 from pTB101 & SV40 early region
RC   pTBAS208 from pTB208 & SV40 early region
RC   pRE3 from pBR322, tet gene
RC   pRE4 from pRE3 & SV40 early gene
RC   pRE7 from pRE4 & linker
RC   pMMB1, pMMB14, pMMB24, pMMB28 from pRE7 & pX3
RC   pRE7BS from pRE7
RC   pMMB1BS from pMMB1 & pRE7BS
RC   pMMB28BS from pMMB28 & pRE7BS
RC   pTCT, pTCTI from pEMP & conalbumin AluI fragment
RC   pSVCT from pHS102 & pTCT
RC   pSVCTI from pHS102 & pTCTI
RC   pTCTB, pTCTBI, pTBCT, pTBICT from pHS102 & pTCT
RC   pTCTB3, pTCTBI3 from pHS102 & pTCT
RC   pEMPB3, pEMPBI3 from pHS102 & pEMP
RC   pTCTB37, pTCTBI37 from pBR322 & pTCT
RC   pEMPB37, pEMPBI37 from pBR322 & pEMP
RC   pHSX102 from pHS102 & conalbumin gene
RC   pSVA500, pSVA40, pSVA29 from pEMP & adenovirus 2 MLP
RC   pSVPB2A500, pSVPB2IA500 from pHS102 & pSVA500
RA   Moreau P., Hen R., Wasylyk B., Everett R., Gaub M.P., Chambon P.;
RT   "The SV40 72 base pair repeat has a striking effect on gene
RT   expression both in SV40 and other chimeric recombinants";
RL   Nucleic Acids Res. 9:6047-6068(1981).
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RN   [3]
RC   pPybetalate+ from beta-globin gene & linker & p.43.34.70, polyoma
RC   pbeta2x from beta-globin gene
RC   pbeta(244+)beta from polyoma virus enhancer & pbeta2x, beta-globin
RA   De Villiers J., Schaffner W.;
RT   "A small segment of polyoma virus DNA enhances the expression of a
RT   cloned beta-globin gene over a distance of 1400 base pairs";
RL   Nucleic Acids Res. 9:6251-6264(1981).
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RN   [4]
RC   pX3 from pBR322 & SV40 early region mutant cs1096
RA   DiMaio D.;
RT   ;
RL   Unpublished (1981).
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RN   [5]
RC   p.43.34.70 from polyoma virus
RA   Tyndall C., Kamen R.;
RT   ;
RL   Unpublished (1981).
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RN   [6]
RC   pPyA2 from polyoma virus
RA   Weissmann C.;
RT   ;
RL   Unpublished (1981).
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CC   Chimeric chicken conalbumin promoter/SV40 early gene recombinants
CC   grown in Hela cells.
CC   The 72-bp repeat (found 5' to the SV40 promoter) is a potentiator
CC   of initiation of transcription from 'tata' box-dependent and
CC   box--independent natural or substitute promoter elements. The
CC   potential proximal promoter sequences are activated in preference to
CC   more distal ones. [1] probed the effectiveness of the 72-bp enhancer
CC   region by inserting it in different positions and orientations to
CC   analyze its effect at the RNA level.
CC   NCBI gi: 207784
CC   NM (pSVCA)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(chicken conalbumin promoter)(SV40 early gene)(pHS102)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. chicken, conalbumin promoter
FT                   -> pHS102
FT                   1. pHS102
FT                   2. SV40, early
FT                   -> pSVCA"
FT   misc_RNA        44..>59
FT                   /note="RNA I"
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SQ   Sequence 59 BP; 12 A; 18 C; 15 G; 14 T; 0 other;
     tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgagcc
//