back Return to this vector's summary.
ID   PSW272     preliminary; circular DNA; SYN; 8700 BP.
AC   ATCC39609;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Vertebrate/E.coli virus vector pSW272 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pSW253 from pBR322 & REV-A
RC   pSW254 from pSW253
RC   plasmid from pBR322 & pSW253 & REV-A provirus
RC   pSW279 from plasmid & pSW210
RC   pSW283 from pSW253 & pSW210
RC   pSW272 from pSW227 & linker
RC   SW272 from pSW272 & SNV-TK
RC   SW279 from pSW279 & SNV-TK
RC   SW283 from pSW283 & SNV-TK
RA   Watanabe S., Temin H.M.;
RT   "Construction of a helper cell line for avian
RT   reticuloendotheliosis virus cloning vectors";
RL   Mol. Cell. Biol. 3:2241-2249(1983).
RN   [2]
RC   pTK23adeltadeltaterR from pSNV-60B & HSV TKdeltaterR
RC   pSW222 from pTK23adeltadeltaterR
RC   pSW210 from pBR322 & pSNV-60B & linker
RC   pSW227 from pSW210 & pSW222
RC   pSW226, pSW249, pSW250, pSW251 from pSW227
RA   Watanabe S., Temin H.M.;
RT   "Encapsidation sequences for spleen necrosis virus, an avian
RT   retrovirus, are between the 5' long terminal repeat and the start
RT   of the gag gene";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:5986-5990(1982).
RN   [3]
RC   pSW272 from pSW227 & linker
RA   Temin H.M.;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,650,764 dated Mar. 17, 1987.
RN   [4]
RC   pTK2, pTK9, pTK19, pTK22, pTK23 from p60BSal & pTK1, HSV tk gene
RC   pTKdeltater(RP) from pTK1
RC   pTK2deltadeltater(RP) from pTKdeltater(RP) & pTK2
RC   SNV TK2deltadeltater(R) from SNV & pTK2deltadeltater(RP)
RA   Shimotohno K., Temin H.M.;
RT   "Formation of infectious progeny virus after insertion of herpes
RT   simplex thymidine kinase gene into DNA of an avian retrovirus";
RL   Cell 26:67-77(1981).
RN   [5]
RC   pREV-T S/H 5', pREV-T Hp/S 3' from pBR322 & REV & SNV
RA   Chen I.S., Temin H.M.;
RT   "Substitution of 5' helper virus sequences into non-rel portion of
RT   reticuloendotheliosis virus strain T suppresses transformation of
RT   chicken spleen cells";
RL   Cell 31:111-120(1982).
RN   [6]
RC   pGT2 from SNV & HSV tk gene
RC   pGT6 from SNV & HSV tk gene
RA   Tarpley W.G.;
RT   ;
RL   Unpublished (1984).
RN   [7]
RC   p60BSal from pBR322 & SNV
RA   O'Rear J.J., Temin H.M.;
RT   "Mapping of alterations in non-infectious proviruses of spleen
RT   necrosis virus";
RL   J. Virol. 39:138-149(1981).
CC   This retrovirus vector is itself replication incompetent and is
CC   designed to be used with a helper cell line (ATCC CRL 8468). [3]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSW272)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (virus)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (avian reticuloendotheliosis virus)(spleen necrosis virus SNV)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pSNV-60B remove BglII-BglII 4800bp
FT                   [1900..6700]
FT                   2. HSV BglII-BglII, TK gene with no polyA/TKdeltaterR
FT                   -> pTK23adeltadeltaterR
FT                   1. pTK23adeltadeltaterR remove XbaI-XbaI 900bp
FT                   [1020..1950]
FT                   -> pSW222 [SNV-TKdeltaterR]
FT                   1. pBR322 SalI
FT                   2. pSNV-60B remove SalI-AvrII 860..7700
FT                   :EcoRI linker
FT                   -> pSW210 [SNV LTR]
FT                   1. pSW210 SalI
FT                   2. pSW222 SalI-SalI, SNV-TKdeltaterR
FT                   -> pSW227
FT                   1. pSW227 XbaI
FT                   BAL31 nuclease
FT                   XbaI linker:XbaI linker
FT                   -> pSW272"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO spleen necrosis virus (SNV) LTR;
FT                   retrovirus"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN vertebrate thymidine kinase gene (TK+)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;