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ID   PSW272     preliminary; circular DNA; SYN; 8700 BP.
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AC   ATCC39609;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Vertebrate/E.coli virus vector pSW272 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSW253 from pBR322 & REV-A
RC   pSW254 from pSW253
RC   plasmid from pBR322 & pSW253 & REV-A provirus
RC   pSW279 from plasmid & pSW210
RC   pSW283 from pSW253 & pSW210
RC   pSW272 from pSW227 & linker
RC   SW272 from pSW272 & SNV-TK
RC   SW279 from pSW279 & SNV-TK
RC   SW283 from pSW283 & SNV-TK
RA   Watanabe S., Temin H.M.;
RT   "Construction of a helper cell line for avian
RT   reticuloendotheliosis virus cloning vectors";
RL   Mol. Cell. Biol. 3:2241-2249(1983).
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RN   [2]
RC   pTK23adeltadeltaterR from pSNV-60B & HSV TKdeltaterR
RC   pSW222 from pTK23adeltadeltaterR
RC   pSW210 from pBR322 & pSNV-60B & linker
RC   pSW227 from pSW210 & pSW222
RC   pSW226, pSW249, pSW250, pSW251 from pSW227
RA   Watanabe S., Temin H.M.;
RT   "Encapsidation sequences for spleen necrosis virus, an avian
RT   retrovirus, are between the 5' long terminal repeat and the start
RT   of the gag gene";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:5986-5990(1982).
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RN   [3]
RC   pSW272 from pSW227 & linker
RA   Temin H.M.;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,650,764 dated Mar. 17, 1987.
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RN   [4]
RC   pTK2, pTK9, pTK19, pTK22, pTK23 from p60BSal & pTK1, HSV tk gene
RC   pTKdeltater(RP) from pTK1
RC   pTK2deltadeltater(RP) from pTKdeltater(RP) & pTK2
RC   SNV TK2deltadeltater(R) from SNV & pTK2deltadeltater(RP)
RA   Shimotohno K., Temin H.M.;
RT   "Formation of infectious progeny virus after insertion of herpes
RT   simplex thymidine kinase gene into DNA of an avian retrovirus";
RL   Cell 26:67-77(1981).
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RN   [5]
RC   pREV-T S/H 5', pREV-T Hp/S 3' from pBR322 & REV & SNV
RA   Chen I.S., Temin H.M.;
RT   "Substitution of 5' helper virus sequences into non-rel portion of
RT   reticuloendotheliosis virus strain T suppresses transformation of
RT   chicken spleen cells";
RL   Cell 31:111-120(1982).
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RN   [6]
RC   pGT2 from SNV & HSV tk gene
RC   pGT6 from SNV & HSV tk gene
RA   Tarpley W.G.;
RT   ;
RL   Unpublished (1984).
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RN   [7]
RC   p60BSal from pBR322 & SNV
RA   O'Rear J.J., Temin H.M.;
RT   "Mapping of alterations in non-infectious proviruses of spleen
RT   necrosis virus";
RL   J. Virol. 39:138-149(1981).
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CC   This retrovirus vector is itself replication incompetent and is
CC   designed to be used with a helper cell line (ATCC CRL 8468). [3]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSW272)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (virus)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (avian reticuloendotheliosis virus)(spleen necrosis virus SNV)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSNV-60B remove BglII-BglII 4800bp
FT                   [1900..6700]
FT                   2. HSV BglII-BglII, TK gene with no polyA/TKdeltaterR
FT                   -> pTK23adeltadeltaterR
FT                   1. pTK23adeltadeltaterR remove XbaI-XbaI 900bp
FT                   [1020..1950]
FT                   -> pSW222 [SNV-TKdeltaterR]
FT                   1. pBR322 SalI
FT                   2. pSNV-60B remove SalI-AvrII 860..7700
FT                   :EcoRI linker
FT                   -> pSW210 [SNV LTR]
FT                   1. pSW210 SalI
FT                   2. pSW222 SalI-SalI, SNV-TKdeltaterR
FT                   -> pSW227
FT                   1. pSW227 XbaI
FT                   BAL31 nuclease
FT                   XbaI linker:XbaI linker
FT                   -> pSW272"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO spleen necrosis virus (SNV) LTR;
FT                   retrovirus"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN vertebrate thymidine kinase gene (TK+)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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