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ID   PSZ219     preliminary; circular DNA; SYN; 1 BP.
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AC   IG7043;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pSZ219 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSZ92 from YRp7
RC   pSZ209 from pSZ92
RC   pSZ210 from pSZ209 & pYeleu10, LEU2 gene
RC   pSZ211 from pSZ210
RC   pSZ212 from pSZ210 & HIS3 gene
RC   pSZ213 from pSZ212
RC   pSZ216 from pSZ213 & Tetrahymena telomere
RC   pT616 from pSZ216
RC   pSZ219, pSZ219-5 from pSZ216 & yeast telomere
RC   pSZ220 from pSZ219-5
RA   Szostak J.W., Blackburn E.H.;
RT   "Cloning yeast telomeres on linear plasmid vectors";
RT   recombination";
RL   Cell 29:245-255(1982).
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RN   [2]
RC   pSZ221 from pBR322 & Tetrahymena telomere
RC   [pSZ222 from pSZ221 & LEU2 gene]
RA   Szostak J.W.;
RT   "Structural requirements for telomere resolution";
RL   Cold Spring Harb. Symp. Quant. Biol. 47:1187-1194(1982).
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CC   NM (pSZ219)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae LL20)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YRp7 remove HindIII-HindIII, TRP1 gene
FT                   -> pSZ92
FT                   1. pSZ92 BglII, ARS1
FT                   sulfite mutagenesis
FT                   -> pSZ209 [ARS1]
FT                   1. pSZ209 SalI
FT                   2. pYeleu10 SalI-XhoI, LEU2 gene
FT                   -> pSZ210
FT                   1. pSZ210 remove short BamHI-SalI
FT                   2. yeast DNA BamHI-SalI, HIS3 gene
FT                   -> pSZ212
FT                   1. pSZ212 BamHI
FT                   fill in
FT                   -> pSZ213 [no BamHI, 1 BglII in HIS3]
FT                   1. pSZ213 BglII
FT                   2. Tetrahymena rDNA BamHI-3'end 1500bp, telomere
FT                   -> pSZ216 [telomere at each end; linear]
FT                   1. pSZ216 PvuI-3'end 7600bp, LEU2/1 telomere
FT                   2. yeast telomere PvuI-3'end 2500bp-2700bp
FT                   -> pSZ219"
FT   misc_feature    0..0
FT                   /note="Tetrahymena CAAA/CCAA/CCCA repeat"
FT   misc_feature    0..0
FT                   /note="Tetrahymena unique rDNA"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   misc_feature    0..0
FT                   /note="yeast Y element, with strong ARS"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuI"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI"
FT   misc_feature    0..0
FT                   /note="Tetrahymena CAAA/CCAA/CCCA repeat"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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