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ID   PT7SCA     preliminary; circular DNA; SYN; 3915 BP.
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AC   IG1901;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT7-SCA - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGP1-1 from pKC30 & T7 phage gene I & linker
RC   pGP1-2 from pGP1-1 & pKB280-cI857 & pACYC177
RC   from pGP1-2 & pT7-1
RC   pGP5-1 from pT7-1 & T7 genes 5/5.3
RC   from pGP1-2 & pGP5-1
RA   Tabor S., Richardson C.C.;
RT   "A bacteriophage T7 RNA polymerase/promoter system for controlled
RT   exclusive expression of specific genes";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:1074-1078(1985).
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RN   [2]
RC   pT7-7Ap from pT7-7
RC   pT7-SCA from pT7- series
RA   ;
RT   ;
RL   Comments 19:14-15(1992).
RL   ., USB, Cleveland OH.
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CC   NM (pT7-SCA)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (non-coding)
CC   TY (phagemid)
CC   SP (USB)
CC   HO (E.coli)
CC   CP ()
CC   FN (transcription)
CC   SE ()
CC   PA (pBR322)
CC   BR (pT7-0)(pT7-1)(pT7-2)(pT7-7)(pT7-7A)(pT7-SCII)(pT7-SC)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. phage T7 150bp, promoter
FT                   -> pT7-7 4500bp
FT                   1. pT7-7
FT                   -> pT7-SCA 3915bp"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli bla gene"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB T1"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB T2"
FT   misc_binding    34..79
FT                   /note="MCS
FT                   HindIII-PstI-SalI-XbaI-BamHI-SmaI-SacI-EcoRI-NdeI"
FT   RBS             complement(0..0)
FT                   /note="RBS bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   terminator      complement(0..0)
FT                   /note="TER E. coli rrnB T2"
FT   terminator      complement(0..0)
FT                   /note="TER E. coli rrnB T1"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli bla gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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