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ID   PTAG4      preliminary; circular DNA; SYN; 4120 BP.
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AC   IG9004;
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DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli phagemid vector pTAG4 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pTAG4 from pUC18 & SV40 early & adenovirus
RA   Krizman D., Berget S.M.;
RT   ;
RL   Focus 15:106-106(1993).
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CC   NM (pTAG4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (BRL)
CC   HO (E.coli)(vertebrate cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp
FT                   2. SV40 HindIII-PvuII 344bp 5172..5243..273, early
FT                   3. adenovirus
FT                   -> pTAG4 4120bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             100..1000
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    392..392
FT                   /note="SIT BcgI"
FT   misc_binding    847..847
FT                   /note="SIT BsaI"
FT   misc_binding    914..914
FT                   /note="SIT Eam1105I"
FT   rep_origin      1000..2000
FT                   /note="ORI phage f1"
FT   promoter        2000..2614
FT                   /note="PRO SV40 early region"
FT   misc_binding    2578..2578
FT                   /note="SIT SfiI"
FT   misc_feature    2614..2727
FT                   /note="adenovirus exon1"
FT   misc_feature    2959..3030
FT                   /note="adenovirus exon2"
FT   misc_binding    3081..3427
FT                   /note="MCS BstEII-Tth111I-BsmI-NspV-EcoRI-AatII-SalI-
FT                   CvnI-SmaI/XmaI-AvaI-ApaI-EcoO109I-BamHI-MunI-BclI-
FT                   BglII-BssHII-MamI/BsaBI-EcoRV-Eco47III-NheI-PinAI-
FT                   HpaI-SnaBI-Bst1107I-Kpn2I-NdeI-NotI-XmaIII-NarI/BbeI-
FT                   MluI-SunI-NruI-SstI-XhoI-SpeI-MscI-AflII"
FT                   replaces mouse thymidine kinase promoter PstI-HindIII
FT                   inserted into HindIII-NsiI of pGEM7"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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