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ID   PTB220     preliminary; circular DNA; SYN; 7600 BP.
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AC   ATCC37317;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pTB220 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pTB199 from YIp5 & YEp24
RC   pTB220 from pTB199
RC   pLP1 from RAD2 gene
RC   pLP8 from pLP1 & YIp5
RC   pLP5 from pLP1
RC   pLP6 from pLP1 & pTB199
RC   pLP7 from pLP6
RC   pLP10 from pLP5 & pTB220
RC   pLP9 from YIp5 & RAD2 gene
RA   Higgins D.R., Prakash L., Reynolds P., Prakash S.;
RT   "Isolation and characterization of the RAD2 gene of Saccharomyces
RT   cerevisiae";
RL   Gene 30:121-128(1984).
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CC   General purpose plasmid vector containing the 2 micron origin.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pTB220)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YIp5 BalI 5541bp 2628..2628
FT                   2. YEp24 EcoRI-EcoRI 2240bp 2..2242, yeast 2 micron
FT                   BAL31 nuclease:BAL31 nuclease
FT                   Klenow:Klenow
FT                   -> pTB199 7600bp [unique EcoRI in pBR322]
FT                   1. pTB199 HindIII 7600bp, YIp5 33..33
FT                   Klenow:Klenow
FT                   -> pTB220 7600bp [unique HindIII in pBR322]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-HindIII-PvuII-ClaI-BamHI-
FT                   SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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