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ID   PTDT1      preliminary; circular DNA; SYN; 7900 BP.
AC   ATCC37255;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pTDT1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   YRp7-K from YRp7
RC   pTRP54 from pYe(trp1/trp5)
RC   pTRP51 from pYe(trp1/trp5) & YRp17
RC   pSV2dhfr from pSV2 & DHFR gene
RC   pTD1 from pTRP51 & pSV2dhfr & linker
RC   pADA1 from pTD1 & pAAH5
RC   pTDT1 from pTD1 & pTRP54
RC   pTD2 from pTD1 & YCp19
RC   pTD4 from pTD1 & pYe(CEN3)30
RC   pTD3 from pTD1 & YEp13
RC   pADA3 from pADA1 & YRp7-K
RC   pADA4 from pADA1 & pYe(CEN3)30
RC   pGDT3 from pBM125 & pTDT1
RC   pADA2 from pADA1 & pUC8
RA   Miyajima A., Miyajima I., Arai K., Arai N.;
RT   "Expression of plasmid R388-encoded type II dihydrofolate
RT   reductase as a dominant selective marker in Saccharomyces
RT   cerevisiae";
RL   Mol. Cell. Biol. 4:407-414(1984).
RN   [2]
RC   pYe(trp5) from pBR322 & TRP5 gene
RA   Zalkin H., Yanofsky C.;
RT   "Yeast gene TRP5: structure, function, regulation";
RL   J. Biol. Chem. 257:1491-1500(1982).
RN   [3]
RC   pYe(trp1/trp5) from pBR322 & TRP1 gene & TRP5 gene
RA   Berg P.;
RT   ;
RL   Unpublished (1982).
CC   Plasmid contains dhfr as a dominant selective marker in Saccharomyces
CC   cerevisiae.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pTDT1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli MC1061)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. yeast, TRP1 gene
FT                   3. yeast, TRP5 gene
FT                   -> pYe(trp1/trp5) 9100bp
FT                   1. pYe(trp1/trp5) BamHI-EcoRI 1300bp, ARS1/TRP1/TRP5
FT                   2. YRp17 BamHI 7002bp 1840..1840, URA3 gene
FT                   -> pTRP51 8000bp
FT                   1. pTRP51 remove small EcoRI-ClaI, in TRP5 gene
FT                   2. pSV2-dhfr EcoRI-HindIII,
FT                   \ pSV2-cat EcoRI = 2619 4752
FT                   :ClaI linker 6bp atcgat
FT                   EcoRI-ClaI, DHFR gene
FT                   -> pTD1 7500bp
FT                   1. pYe(trp1/trp5) TaqI-EcoRI 400bp, TRP5 terminator
FT                   2. pUC8 remove EcoRI-AccI 17bp 231..248, MCS/2648bp
FT                   -> pTRP54 3200bp
FT                   1. pTD1 EcoRI 7500bp, DHFR gene
FT                   Klenow:Klenow
FT                   2. pTRP54 EcoRI-HindIII 3200bp 261..3200..231
FT                   Klenow:Klenow
FT                   -> pTDT1 7900bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   promoter        0..0
FT                   /note="PRO yeast TRP5 gene"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="ANT yeast DHFR gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;